Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates

Vincent M. Edwards, James R. Deringer, Scott D. Callantine, Claudia F. Deobald, Philip H. Berger, Vivek Kapur, Cynthia V. Stauffacher, Gregory A. Bohach

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The type C staphylococcal enterotoxins (SECs) are a group of highly conserved proteins with substantial antigenic cross-reactivity. Although Staphylococcus intermedius and coagulase-positive species of staphylococci are reported to produce SEC and other SEs, toxins produced by species other than Staphylococcus aureus have not been previously characterized. In this study we report the molecular, biological, and immunological properties of the canine SEC (SEC(canine)) expressed by pathogenic isolates of S. intermedius. The mature form of SEC(canine) has 239 amino acid residues and a pI of 7.0. Typical of the SEs, purified SEC(canine) induces an emetic response in monkeys and the proliferation of T cells in a Vβ-dependent manner. Although SEC(canine) has > 95% sequence identity to previously described SEC variants, its sequence is most related to SEC2 and SEC3. In contrast to the sequence similarity, the Vβ profile induced by SEC(canine) is typical of that induced by SEC1. This result is likely explained by the conservation of a cysteine residue at position 26 in SEC(canine); residues at this position have been previously shown to determine subtype-dependent differences in T-cell receptor interactions of other SEs. Overall, these results show that superantigen toxins produced by the multiple members of the genus Staphylococcus are highly conserved in respect to biological and structural properties. Further, the frequent association of SEC(canine) with pyoderma in dogs supports the notion that the toxins are important for staphylococcal survival and pathogenesis.

Original languageEnglish (US)
Pages (from-to)2346-2352
Number of pages7
JournalInfection and Immunity
Volume65
Issue number6
StatePublished - Jun 18 1997

Fingerprint

Staphylococcus intermedius
Pyoderma
Enterotoxins
Canidae
Staphylococcus
Emetics
Superantigens
Coagulase
T-Cell Antigen Receptor
Cell Communication
Haplorhini
Cysteine
Staphylococcus aureus
Dogs
T-Lymphocytes
Amino Acids

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Edwards, V. M., Deringer, J. R., Callantine, S. D., Deobald, C. F., Berger, P. H., Kapur, V., ... Bohach, G. A. (1997). Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates. Infection and Immunity, 65(6), 2346-2352.
Edwards, Vincent M. ; Deringer, James R. ; Callantine, Scott D. ; Deobald, Claudia F. ; Berger, Philip H. ; Kapur, Vivek ; Stauffacher, Cynthia V. ; Bohach, Gregory A. / Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates. In: Infection and Immunity. 1997 ; Vol. 65, No. 6. pp. 2346-2352.
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abstract = "The type C staphylococcal enterotoxins (SECs) are a group of highly conserved proteins with substantial antigenic cross-reactivity. Although Staphylococcus intermedius and coagulase-positive species of staphylococci are reported to produce SEC and other SEs, toxins produced by species other than Staphylococcus aureus have not been previously characterized. In this study we report the molecular, biological, and immunological properties of the canine SEC (SEC(canine)) expressed by pathogenic isolates of S. intermedius. The mature form of SEC(canine) has 239 amino acid residues and a pI of 7.0. Typical of the SEs, purified SEC(canine) induces an emetic response in monkeys and the proliferation of T cells in a Vβ-dependent manner. Although SEC(canine) has > 95{\%} sequence identity to previously described SEC variants, its sequence is most related to SEC2 and SEC3. In contrast to the sequence similarity, the Vβ profile induced by SEC(canine) is typical of that induced by SEC1. This result is likely explained by the conservation of a cysteine residue at position 26 in SEC(canine); residues at this position have been previously shown to determine subtype-dependent differences in T-cell receptor interactions of other SEs. Overall, these results show that superantigen toxins produced by the multiple members of the genus Staphylococcus are highly conserved in respect to biological and structural properties. Further, the frequent association of SEC(canine) with pyoderma in dogs supports the notion that the toxins are important for staphylococcal survival and pathogenesis.",
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Edwards, VM, Deringer, JR, Callantine, SD, Deobald, CF, Berger, PH, Kapur, V, Stauffacher, CV & Bohach, GA 1997, 'Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates', Infection and Immunity, vol. 65, no. 6, pp. 2346-2352.

Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates. / Edwards, Vincent M.; Deringer, James R.; Callantine, Scott D.; Deobald, Claudia F.; Berger, Philip H.; Kapur, Vivek; Stauffacher, Cynthia V.; Bohach, Gregory A.

In: Infection and Immunity, Vol. 65, No. 6, 18.06.1997, p. 2346-2352.

Research output: Contribution to journalArticle

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T1 - Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates

AU - Edwards, Vincent M.

AU - Deringer, James R.

AU - Callantine, Scott D.

AU - Deobald, Claudia F.

AU - Berger, Philip H.

AU - Kapur, Vivek

AU - Stauffacher, Cynthia V.

AU - Bohach, Gregory A.

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N2 - The type C staphylococcal enterotoxins (SECs) are a group of highly conserved proteins with substantial antigenic cross-reactivity. Although Staphylococcus intermedius and coagulase-positive species of staphylococci are reported to produce SEC and other SEs, toxins produced by species other than Staphylococcus aureus have not been previously characterized. In this study we report the molecular, biological, and immunological properties of the canine SEC (SEC(canine)) expressed by pathogenic isolates of S. intermedius. The mature form of SEC(canine) has 239 amino acid residues and a pI of 7.0. Typical of the SEs, purified SEC(canine) induces an emetic response in monkeys and the proliferation of T cells in a Vβ-dependent manner. Although SEC(canine) has > 95% sequence identity to previously described SEC variants, its sequence is most related to SEC2 and SEC3. In contrast to the sequence similarity, the Vβ profile induced by SEC(canine) is typical of that induced by SEC1. This result is likely explained by the conservation of a cysteine residue at position 26 in SEC(canine); residues at this position have been previously shown to determine subtype-dependent differences in T-cell receptor interactions of other SEs. Overall, these results show that superantigen toxins produced by the multiple members of the genus Staphylococcus are highly conserved in respect to biological and structural properties. Further, the frequent association of SEC(canine) with pyoderma in dogs supports the notion that the toxins are important for staphylococcal survival and pathogenesis.

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Edwards VM, Deringer JR, Callantine SD, Deobald CF, Berger PH, Kapur V et al. Characterization of the canine type c enterotoxin produced by Staphylococcus intermedius pyoderma isolates. Infection and Immunity. 1997 Jun 18;65(6):2346-2352.