Characterization of the CCK-B/gastrin-like receptor in human colon cancer

Jill P. Smith, Elizabeth A. Stock, Michael G. Wotring, Patricia McLaughlin, Ian Zagon

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The gastrointestinal peptide, gastrin, tonically stimulates growth of human colon cancer cells in vivo and in vitro, and does so in a receptor- mediated fashion. This study defined the nature of gastrin binding in human colon cancer using [3H]L-365,260, a specific cholecystokinin B (CCK- B)/gastrin antagonist found to block gastrin's effects on growth. Following elucidation of optimal binding conditions (e.g., pH, time, and temperature) in log phase HT-29 human colon cancer cells, specific and saturable binding with a dissociation constant of 4.8 ± 0.7 nM and a maximal binding capacity (B(max)) of 320 ± 120 fmol/mg protein, consistent with a single binding site, was recorded. Binding was localized to the membrane fraction. Exposure to gastrin or receptor antagonist decreased and increased, respectively, the B(max). Competition experiments indicated that L-365,260 was 25- and 200- fold more effective at displacing radiolabeled L-365,260 than gastrin and cholecystokinin, respectively. In contrast to log phase cells, the B(max) was decreased by 67 to 76% in confluent and postconfluent cultures. Binding activity was observed in other cell lines examined, as well as in xenografts and colon cancers obtained at surgery. Binding in normal human colonic mucosa was 10-fold less than in colon cancer. These results provide the first comprehensive identification and characterization of a CCK-B/gastrin-like receptor in human colon cancer.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume271
Issue number3 40-3
StatePublished - Sep 1 1996

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Cholecystokinin B Receptor
Colonic Neoplasms
Gastrins
Cholecystokinin
Growth
Heterografts
Mucous Membrane
Binding Sites
Cell Line
Peptides
Temperature
Membranes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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title = "Characterization of the CCK-B/gastrin-like receptor in human colon cancer",
abstract = "The gastrointestinal peptide, gastrin, tonically stimulates growth of human colon cancer cells in vivo and in vitro, and does so in a receptor- mediated fashion. This study defined the nature of gastrin binding in human colon cancer using [3H]L-365,260, a specific cholecystokinin B (CCK- B)/gastrin antagonist found to block gastrin's effects on growth. Following elucidation of optimal binding conditions (e.g., pH, time, and temperature) in log phase HT-29 human colon cancer cells, specific and saturable binding with a dissociation constant of 4.8 ± 0.7 nM and a maximal binding capacity (B(max)) of 320 ± 120 fmol/mg protein, consistent with a single binding site, was recorded. Binding was localized to the membrane fraction. Exposure to gastrin or receptor antagonist decreased and increased, respectively, the B(max). Competition experiments indicated that L-365,260 was 25- and 200- fold more effective at displacing radiolabeled L-365,260 than gastrin and cholecystokinin, respectively. In contrast to log phase cells, the B(max) was decreased by 67 to 76{\%} in confluent and postconfluent cultures. Binding activity was observed in other cell lines examined, as well as in xenografts and colon cancers obtained at surgery. Binding in normal human colonic mucosa was 10-fold less than in colon cancer. These results provide the first comprehensive identification and characterization of a CCK-B/gastrin-like receptor in human colon cancer.",
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Characterization of the CCK-B/gastrin-like receptor in human colon cancer. / Smith, Jill P.; Stock, Elizabeth A.; Wotring, Michael G.; McLaughlin, Patricia; Zagon, Ian.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 271, No. 3 40-3, 01.09.1996.

Research output: Contribution to journalArticle

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