Characterization of the group 1 and group 2 sigma factors of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus

Tanja M. Gruber, Donald A. Bryant

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Abstract

The group 1 and group 2 σ70-type sigma factors of the green sulfur bacterium Chlorobium tepidum and of the green sulfur bacterium Chloroflexus aurantiacus were cloned and characterized. Cb. tepidum was found to contain one σ70-type sigma factor; the expression of the gene wets analyzed by Northern blot hybridization and primer-extension mapping. Cf. aurantiacus has genes encoding four sigma factors of groups 1 and 2. The expression of these genes was examined in cells grown aerobically and anaerobically. The sigC gene was expressed at approximately equal levels under both conditions, resulting in its designation as the group 1 sigma factor of this organism. The only other detectable transcripts arose from the sigB gene, which was expressed at higher levels during aerobic growth. A phylogenetic tree was obtained using the group 1 sigma factors of Cb. tepidum, Cf. aurantiacus, and diverse eubacteria as the molecular marker. The resulting phylogenetic tree shows that Cb. tepidum and Cf. aurantiacus are related to each other and to the cyanobacteria. The relationship of the group 2 sigma factors of Cf. aurantiacus and the cyanobacteria was more specifically examined phylogenetically. The group 2 sigma factors of Cf. aurantiacus probably arose by gene duplication events after the split of the green sulfur bacteria from other photosynthetic eubacteria.

Original languageEnglish (US)
Pages (from-to)285-296
Number of pages12
JournalArchives of Microbiology
Volume170
Issue number4
DOIs
StatePublished - Sep 30 1998

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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