Characterization of the thymus leukemia (TL) product encoded by the BALB/c T3(c) gene by DNA-mediated gene transfer. Comparison to the T13(c) product and BALB/c leukemia TL

Michael J. Chorney, H. Mashimo, Y. Bushkin, S. G. Nathenson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Using DNA-mediated gene transfer, we have studied the TL protein products encoded by both the T3(c) and T13(c) BALB/c genes. Biochemically, the proteins differed in their mw. and pI points; serologically, although both molecules were recognized by TL alloantiserum, only the T13(c) protein was recognized by monoclonal TL antibodies. Interestingly, both proteins were serologically and immunochemically recognized by leukemia-specific TL.4 antiserum. The quantity of cell surface T13 was significantly greater than T3 possibly due to the less efficient splicing of T3 transcripts in the L cell nucleus; both genes directed the synthesis of cytoplasmic RNA containing an unspliced intron 3 as assessed by S1 analysis. In toto, the results suggest that T3(c) is similar or perhaps identical with the novel TL product previously identified on the surface of certain x-ray-induced BALB/c leukemias.

Original languageEnglish (US)
Pages (from-to)3762-3768
Number of pages7
JournalJournal of Immunology
Volume143
Issue number11
StatePublished - Jan 1 1989

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Thymus Gland
Leukemia
DNA
Genes
Proteins
Cell Nucleus
Introns
Immune Sera
X-Rays
RNA
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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abstract = "Using DNA-mediated gene transfer, we have studied the TL protein products encoded by both the T3(c) and T13(c) BALB/c genes. Biochemically, the proteins differed in their mw. and pI points; serologically, although both molecules were recognized by TL alloantiserum, only the T13(c) protein was recognized by monoclonal TL antibodies. Interestingly, both proteins were serologically and immunochemically recognized by leukemia-specific TL.4 antiserum. The quantity of cell surface T13 was significantly greater than T3 possibly due to the less efficient splicing of T3 transcripts in the L cell nucleus; both genes directed the synthesis of cytoplasmic RNA containing an unspliced intron 3 as assessed by S1 analysis. In toto, the results suggest that T3(c) is similar or perhaps identical with the novel TL product previously identified on the surface of certain x-ray-induced BALB/c leukemias.",
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Characterization of the thymus leukemia (TL) product encoded by the BALB/c T3(c) gene by DNA-mediated gene transfer. Comparison to the T13(c) product and BALB/c leukemia TL. / Chorney, Michael J.; Mashimo, H.; Bushkin, Y.; Nathenson, S. G.

In: Journal of Immunology, Vol. 143, No. 11, 01.01.1989, p. 3762-3768.

Research output: Contribution to journalArticle

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AU - Chorney, Michael J.

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AB - Using DNA-mediated gene transfer, we have studied the TL protein products encoded by both the T3(c) and T13(c) BALB/c genes. Biochemically, the proteins differed in their mw. and pI points; serologically, although both molecules were recognized by TL alloantiserum, only the T13(c) protein was recognized by monoclonal TL antibodies. Interestingly, both proteins were serologically and immunochemically recognized by leukemia-specific TL.4 antiserum. The quantity of cell surface T13 was significantly greater than T3 possibly due to the less efficient splicing of T3 transcripts in the L cell nucleus; both genes directed the synthesis of cytoplasmic RNA containing an unspliced intron 3 as assessed by S1 analysis. In toto, the results suggest that T3(c) is similar or perhaps identical with the novel TL product previously identified on the surface of certain x-ray-induced BALB/c leukemias.

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