Characterization of the yeast copper-inducible promoter system in Arabidopsis thaliana

C. L. Granger, R. J. Cyr

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Abstract

Inducible promoters or gene-switches are used to both spatially and temporally regulate gene expression. Such regulation can provide information concerning the function of a gene in a developmental context as well as avoid potential harmful effects due to overexpression. A gfp construct under the control of a copper-inducible promoter was introduced into Arabidopsis thaliana (L.) Heynh. and the regulatory parameters of this inducible promoter were determined. Here, we describe the time-course of up- and down-regulation of GFP expression in response to copper level, the optimal regulatory levels of copper, and the tissue specificity of expression in three transgenic lines. We conclude that the copper-inducible promoter system may be useful in regulating the time and location of gene expression in A. thaliana.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalPlant Cell Reports
Volume20
Issue number3
DOIs
Publication statusPublished - May 2 2001

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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