Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus

Tibor Pechan, Binghua Jiang, David Steckler, Lijun Ye, Lei Lin, Dawn S. Luthe, W. Paul Williams

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.)resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones were isolated and sequenced. A cDNA clone encoding the 33 kDa cysteine proteinase, mir1, was identified. Two additional clones, mir2 and mir3, encoding putative cysteine proteinases were also identified, mir2 and mir3 fire distinct from mir1 and each other, but show a high degree of homology. All of the mir cDNA clones map to distinct sites on the maize genome. Amino acid sequences encoded by the mir clones are similar to other known cysteine proteinases and are most closely related to the oryzain-α and -β precursors. The ERFNIN motif and a 12 amino acid conserved sequence are present in the propeptide region of the putative proteinases encoded by mir clones, mir2 and mir3 appear to have C-terminal extensions. The phylogenetic tree of nucleotide sequences of mir1, mir2, mir3 and other representative cysteine proteinases from protozoa, plants and animals was constructed.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalPlant molecular biology
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Cysteine Proteases
cysteine proteinases
Bony Callus
Zea mays
callus
Complementary DNA
Clone Cells
clones
corn
Amino Acid Sequence
Peptide Hydrolases
proteinases
amino acid sequences
Spodoptera frugiperda
conserved sequences
Conserved Sequence
oligonucleotides
Gene Library
Oligonucleotides
cDNA libraries

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Pechan, T., Jiang, B., Steckler, D., Ye, L., Lin, L., Luthe, D. S., & Williams, W. P. (1999). Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus. Plant molecular biology, 40(1), 111-119. https://doi.org/10.1023/A:1026494813936
Pechan, Tibor ; Jiang, Binghua ; Steckler, David ; Ye, Lijun ; Lin, Lei ; Luthe, Dawn S. ; Williams, W. Paul. / Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus. In: Plant molecular biology. 1999 ; Vol. 40, No. 1. pp. 111-119.
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Pechan, T, Jiang, B, Steckler, D, Ye, L, Lin, L, Luthe, DS & Williams, WP 1999, 'Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus', Plant molecular biology, vol. 40, no. 1, pp. 111-119. https://doi.org/10.1023/A:1026494813936

Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus. / Pechan, Tibor; Jiang, Binghua; Steckler, David; Ye, Lijun; Lin, Lei; Luthe, Dawn S.; Williams, W. Paul.

In: Plant molecular biology, Vol. 40, No. 1, 01.01.1999, p. 111-119.

Research output: Contribution to journalArticle

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