Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium

Andrawis Andrawis, Elizabeth A. Pease, I. ching Kuan, Erika Holzbaur, Ming Tien

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Two cDNA clones encoding lignin peroxidase isozymes from Phanerochaete chrysosporium have been isolated and characterized. One of the clones, λML-4, encodes isozyme H8 as does the previously reported clone λML-1 [Tien, M. and Tu, C.-P.D. Nature 326 (1987) 520-523; 328, 742]. Our data are consistent with λML-1 and λML-4 being allelic variants. The other clone, λML-5, encodes a homologous isozyme. We have also isolated the genomic clone corresponding to λML-4 cDNA. Conserved residues thought to be essential for peroxidase function were identified in the predicted amino acid sequences of both cDNA clones. Northern blot analyses indicate that these isozymes are expressed during secondary metabolism, appearing on day 4 of growth and increasing on days 5 and 6.

Original languageEnglish (US)
Pages (from-to)673-680
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number2
DOIs
StatePublished - Jul 31 1989

Fingerprint

Phanerochaete
Isoenzymes
Clone Cells
Complementary DNA
Peroxidase
Secondary Metabolism
Amino Acids
Northern Blotting
lignin peroxidase
Amino Acid Sequence
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Andrawis, Andrawis ; Pease, Elizabeth A. ; Kuan, I. ching ; Holzbaur, Erika ; Tien, Ming. / Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 162, No. 2. pp. 673-680.
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abstract = "Two cDNA clones encoding lignin peroxidase isozymes from Phanerochaete chrysosporium have been isolated and characterized. One of the clones, λML-4, encodes isozyme H8 as does the previously reported clone λML-1 [Tien, M. and Tu, C.-P.D. Nature 326 (1987) 520-523; 328, 742]. Our data are consistent with λML-1 and λML-4 being allelic variants. The other clone, λML-5, encodes a homologous isozyme. We have also isolated the genomic clone corresponding to λML-4 cDNA. Conserved residues thought to be essential for peroxidase function were identified in the predicted amino acid sequences of both cDNA clones. Northern blot analyses indicate that these isozymes are expressed during secondary metabolism, appearing on day 4 of growth and increasing on days 5 and 6.",
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Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium. / Andrawis, Andrawis; Pease, Elizabeth A.; Kuan, I. ching; Holzbaur, Erika; Tien, Ming.

In: Biochemical and Biophysical Research Communications, Vol. 162, No. 2, 31.07.1989, p. 673-680.

Research output: Contribution to journalArticle

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