Two cDNA clones encoding lignin peroxidase isozymes from Phanerochaete chrysosporium have been isolated and characterized. One of the clones, λML-4, encodes isozyme H8 as does the previously reported clone λML-1 [Tien, M. and Tu, C.-P.D. Nature 326 (1987) 520-523; 328, 742]. Our data are consistent with λML-1 and λML-4 being allelic variants. The other clone, λML-5, encodes a homologous isozyme. We have also isolated the genomic clone corresponding to λML-4 cDNA. Conserved residues thought to be essential for peroxidase function were identified in the predicted amino acid sequences of both cDNA clones. Northern blot analyses indicate that these isozymes are expressed during secondary metabolism, appearing on day 4 of growth and increasing on days 5 and 6.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 31 1989|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology