Characterizing mycoviruses

Mahtab Peyambari, Marilyn J. Roossinck

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A variety of methods for the detection and characterization of fungal viruses are available. For many years, serological and biological assays were used for virus detection. Today, more sensitive methods like polymerase chain reaction, together with sequencing, are widely used to study viruses. Extracting double-stranded (ds) RNA can be a useful approach to detect and study mycoviruses from fungal tissues, as dsRNAs accumulate in infected cells as copies of viral genomes or as replicative intermediates of single-stranded RNA genomes. Here we present a basic protocol for growing fungal strains and isolating dsRNA using cellulose chromatography, followed by molecular diagnostic methods including cDNA synthesis, sequencing, and determination of 5′ ends by primer ligation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages13-24
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1848
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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