Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Johan Dunevall, Hoda Fathali, Neda Najafinobar, Jelena Lovric, Joakim Wigström, Ann Sofie Cans, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

121 Scopus citations

Abstract

We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.

Original languageEnglish (US)
Pages (from-to)4344-4346
Number of pages3
JournalJournal of the American Chemical Society
Volume137
Issue number13
DOIs
StatePublished - Apr 8 2015

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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