Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Xiaoping Bao, Xiaojun Lian, Kaitlin K. Dunn, Mengxuan Shi, Tianxiao Han, Tongcheng Qian, Vijesh J. Bhute, Scott G. Canfield, Sean P. Palecek

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34. + CD31. + endothelial progenitors that can be purified to >. 95% CD34. + cells via magnetic activated cell sorting (MACS). These CD34. + progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.

Original languageEnglish (US)
Pages (from-to)122-129
Number of pages8
JournalStem Cell Research
Volume15
Issue number1
DOIs
StatePublished - Jul 1 2015

Fingerprint

Pluripotent Stem Cells
Albumins
Endothelial Cells
Therapeutic Human Experimentation
Glycogen Synthase Kinase 3
Eagles
Serum-Free Culture Media
von Willebrand Factor
Ascorbic Acid
Blood Vessels
Culture Media
Intercellular Signaling Peptides and Proteins
Phenotype
Research
Endothelial Progenitor Cells

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Bao, Xiaoping ; Lian, Xiaojun ; Dunn, Kaitlin K. ; Shi, Mengxuan ; Han, Tianxiao ; Qian, Tongcheng ; Bhute, Vijesh J. ; Canfield, Scott G. ; Palecek, Sean P. / Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells. In: Stem Cell Research. 2015 ; Vol. 15, No. 1. pp. 122-129.
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Bao, X, Lian, X, Dunn, KK, Shi, M, Han, T, Qian, T, Bhute, VJ, Canfield, SG & Palecek, SP 2015, 'Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells', Stem Cell Research, vol. 15, no. 1, pp. 122-129. https://doi.org/10.1016/j.scr.2015.05.004

Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells. / Bao, Xiaoping; Lian, Xiaojun; Dunn, Kaitlin K.; Shi, Mengxuan; Han, Tianxiao; Qian, Tongcheng; Bhute, Vijesh J.; Canfield, Scott G.; Palecek, Sean P.

In: Stem Cell Research, Vol. 15, No. 1, 01.07.2015, p. 122-129.

Research output: Contribution to journalArticle

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