Chemically Modified Dendritic Starch: A Novel Nanomaterial for siRNA Delivery

Sarah A. Engelberth, Nadine Hempel, Magnus Bergkvist

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nanostructured starches are naturally derived nanomaterials that can be chemically modified to allow for the introduction of functional groups, enhancing their potential for drug delivery and other biotechnology applications. In this proof of concept study, we investigate chemically modified, enzymatically synthesized glycogen (ESG) nanodendrites as a biodegradable, biocompatible, siRNA delivery system. Commercially available ESG was modified using glycidyltrimethylammonium chloride (GTMA), introducing quaternary ammonium groups via an epoxide ring opening reaction. This cationic ESG (cESG) electrostatically bound siRNA and successfully knocked down protein expression in an in vitro ovarian clear cell carcinoma model. The construct exhibited sustained siRNA delivery for up to 6 days while exhibiting less toxicity than a common liposome-based siRNA delivery reagent, Lipofectamine RNAiMAX. These promising results set the stage for the use of dendritic starch as a cost-effective, easily modifiable nanoscale delivery system for a diverse range of cargo including nucleic acids and therapeutic compounds.

Original languageEnglish (US)
Pages (from-to)1766-1774
Number of pages9
JournalBioconjugate Chemistry
Volume26
Issue number8
DOIs
StatePublished - Aug 19 2015

Fingerprint

Nanostructures
Starch
Nanostructured materials
Small Interfering RNA
Glycogen
Liposomes
Nucleic acids
Biotechnology
Drug delivery
Functional groups
Toxicity
Epoxy Compounds
Ammonium Compounds
Cells
Nucleic Acids
Proteins
Carcinoma
Costs and Cost Analysis
Costs
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Engelberth, Sarah A. ; Hempel, Nadine ; Bergkvist, Magnus. / Chemically Modified Dendritic Starch : A Novel Nanomaterial for siRNA Delivery. In: Bioconjugate Chemistry. 2015 ; Vol. 26, No. 8. pp. 1766-1774.
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Chemically Modified Dendritic Starch : A Novel Nanomaterial for siRNA Delivery. / Engelberth, Sarah A.; Hempel, Nadine; Bergkvist, Magnus.

In: Bioconjugate Chemistry, Vol. 26, No. 8, 19.08.2015, p. 1766-1774.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemically Modified Dendritic Starch

T2 - A Novel Nanomaterial for siRNA Delivery

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AU - Hempel, Nadine

AU - Bergkvist, Magnus

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