Abstract
We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4169-4176 |
| Number of pages | 8 |
| Journal | Molecular Biology Reports |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2012 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
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Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea. / Yang, Jinghua; Liu, Shan; Yang, Xiaodong; Zhang, Mingfang.
In: Molecular Biology Reports, Vol. 39, No. 4, 01.04.2012, p. 4169-4176.Research output: Contribution to journal › Article
TY - JOUR
T1 - Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea
AU - Yang, Jinghua
AU - Liu, Shan
AU - Yang, Xiaodong
AU - Zhang, Mingfang
PY - 2012/4/1
Y1 - 2012/4/1
N2 - We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.
AB - We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.
UR - http://www.scopus.com/inward/record.url?scp=84862980936&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862980936&partnerID=8YFLogxK
U2 - 10.1007/s11033-011-1200-9
DO - 10.1007/s11033-011-1200-9
M3 - Article
C2 - 21773942
AN - SCOPUS:84862980936
VL - 39
SP - 4169
EP - 4176
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
IS - 4
ER -