Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea

Jinghua Yang, Shan Liu, Xiaodong Yang, Mingfang Zhang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.

Original languageEnglish (US)
Pages (from-to)4169-4176
Number of pages8
JournalMolecular Biology Reports
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2012

Fingerprint

Mustard Plant
Male Infertility
Chloroplasts
Hydroxyl Radical
Hydrogen Peroxide
Wounds and Injuries
Genes
Seedlings
Oxidation-Reduction
Oxidative Stress
Homeostasis
Gene Expression
Light
Mitochondrial Genes
Plant Cells
Citric Acid
Iron
Antioxidants

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{02309d5b4816439f98bf92951331b97c,
title = "Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea",
abstract = "We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.",
author = "Jinghua Yang and Shan Liu and Xiaodong Yang and Mingfang Zhang",
year = "2012",
month = "4",
day = "1",
doi = "10.1007/s11033-011-1200-9",
language = "English (US)",
volume = "39",
pages = "4169--4176",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "4",

}

Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea. / Yang, Jinghua; Liu, Shan; Yang, Xiaodong; Zhang, Mingfang.

In: Molecular Biology Reports, Vol. 39, No. 4, 01.04.2012, p. 4169-4176.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea

AU - Yang, Jinghua

AU - Liu, Shan

AU - Yang, Xiaodong

AU - Zhang, Mingfang

PY - 2012/4/1

Y1 - 2012/4/1

N2 - We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.

AB - We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H2O2 and .OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H2O2, not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.

UR - http://www.scopus.com/inward/record.url?scp=84862980936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862980936&partnerID=8YFLogxK

U2 - 10.1007/s11033-011-1200-9

DO - 10.1007/s11033-011-1200-9

M3 - Article

C2 - 21773942

AN - SCOPUS:84862980936

VL - 39

SP - 4169

EP - 4176

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 4

ER -