TY - JOUR
T1 - Choice of laboratory tissue homogenizers matters when recovering nucleic acid from medically important ticks
AU - Jones, Amanda M.
AU - Van De Wyngaerde, Marshall T.
AU - Machtinger, Erika T.
AU - Rajotte, Edwin G.
AU - Baker, Thomas C.
N1 - Funding Information:
This study was funded by the Department of Defense (DoD), Defense Health Program (D6.7-14-C2-I-14-J9-1052) as an effort to evaluate commercially available products for Department of Defense use, and all experiments were conducted at the Walter Reed Army Institute of Research located in Silver Spring, MD, in the Vector Diagnostics Department under the Entomology Branch. Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense.
Publisher Copyright:
© 2020 The Author(s).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Ticks can vector and transmit many pathogens and pose a serious human health threat throughout the world. After collection, many diagnostic laboratories must mechanically disrupt tick specimens for diagnostic testing and research purposes, but few studies have evaluated how well-commercial tissue homogenizers perform this task. We evaluated four commercially available tissue homogenizers: The Bead Ruptor 24 Elite, the Bullet Blender Storm, the gentleMACS Dissociator, and the Precellys 24. We quantitatively compared maceration level, nucleic acid quality, quantity, amplification, and DNA shearing to determine which machines performed the best. The Bead Ruptor 24 Elite had the highest overall score when disrupting a single, uninfected adult Amblyomma americanum (Linnaeus) (Ixodida: Ixodidae) and performed well in follow-on tests including disrupting individual juvenile samples and detecting pathogens from infected samples.
AB - Ticks can vector and transmit many pathogens and pose a serious human health threat throughout the world. After collection, many diagnostic laboratories must mechanically disrupt tick specimens for diagnostic testing and research purposes, but few studies have evaluated how well-commercial tissue homogenizers perform this task. We evaluated four commercially available tissue homogenizers: The Bead Ruptor 24 Elite, the Bullet Blender Storm, the gentleMACS Dissociator, and the Precellys 24. We quantitatively compared maceration level, nucleic acid quality, quantity, amplification, and DNA shearing to determine which machines performed the best. The Bead Ruptor 24 Elite had the highest overall score when disrupting a single, uninfected adult Amblyomma americanum (Linnaeus) (Ixodida: Ixodidae) and performed well in follow-on tests including disrupting individual juvenile samples and detecting pathogens from infected samples.
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U2 - 10.1093/jme/tjaa006
DO - 10.1093/jme/tjaa006
M3 - Article
C2 - 31971588
AN - SCOPUS:85087531854
VL - 57
SP - 1221
EP - 1227
JO - Journal of Medical Entomology
JF - Journal of Medical Entomology
SN - 0022-2585
IS - 4
ER -
