Choline and choline metabolite patterns and associations in blood and milk during lactation in dairy cows

Virginia M. Artegoitia, Jesse L. Middleton, Federico Miguel Harte, Shawn R. Campagna, Michael J. De Veth

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in early, mid and late lactation and analyzed for acetylcholine, free choline, betaine, glycerophosphocholine, lysophosphatidylcholine, phosphatidylcholine, phosphocholine and sphingomyelin using hydrophilic interaction liquid chromatography-tandem mass spectrometry, and quantified using stable isotope-labeled internal standards. Total choline concentration in plasma, which was almost entirely phosphatidylcholine, increased 10-times from early to late lactation (1305 to 13,535 μmol/L). In milk, phosphocholine was the main metabolite in early lactation (492 μmol/L), which is a similar concentration to that found in human milk, however, phosphocholine concentration decreased exponentially through lactation to 43 μmol/L in late lactation. In contrast, phosphatidylcholine was the main metabolite in mid and late lactation (188 μmol/L and 659 μmol/L, respectively), with the increase through lactation positively correlated with phosphatidylcholine in plasma (R2 = 0.78). Unlike previously reported with human milk we found no correlation between plasma free choline concentration and milk choline metabolites. The changes in pattern of phosphocholine and phosphatidylcholine in milk through lactation observed in the bovine suggests that it is possible to manufacture infant formula that more closely matches these metabolites profile in human milk.

Original languageEnglish (US)
Article numbere103412
JournalPloS one
Volume9
Issue number8
DOIs
StatePublished - Aug 26 2014

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Dairies
choline
Metabolites
Choline
Lactation
Milk
Blood
dairy cows
lactation
metabolites
milk
Phosphatidylcholines
phosphatidylcholines
Phosphorylcholine
late lactation
blood
breast milk
Human Milk
Infant Formula
infant formulas

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Artegoitia, Virginia M. ; Middleton, Jesse L. ; Harte, Federico Miguel ; Campagna, Shawn R. ; De Veth, Michael J. / Choline and choline metabolite patterns and associations in blood and milk during lactation in dairy cows. In: PloS one. 2014 ; Vol. 9, No. 8.
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abstract = "Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in early, mid and late lactation and analyzed for acetylcholine, free choline, betaine, glycerophosphocholine, lysophosphatidylcholine, phosphatidylcholine, phosphocholine and sphingomyelin using hydrophilic interaction liquid chromatography-tandem mass spectrometry, and quantified using stable isotope-labeled internal standards. Total choline concentration in plasma, which was almost entirely phosphatidylcholine, increased 10-times from early to late lactation (1305 to 13,535 μmol/L). In milk, phosphocholine was the main metabolite in early lactation (492 μmol/L), which is a similar concentration to that found in human milk, however, phosphocholine concentration decreased exponentially through lactation to 43 μmol/L in late lactation. In contrast, phosphatidylcholine was the main metabolite in mid and late lactation (188 μmol/L and 659 μmol/L, respectively), with the increase through lactation positively correlated with phosphatidylcholine in plasma (R2 = 0.78). Unlike previously reported with human milk we found no correlation between plasma free choline concentration and milk choline metabolites. The changes in pattern of phosphocholine and phosphatidylcholine in milk through lactation observed in the bovine suggests that it is possible to manufacture infant formula that more closely matches these metabolites profile in human milk.",
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Choline and choline metabolite patterns and associations in blood and milk during lactation in dairy cows. / Artegoitia, Virginia M.; Middleton, Jesse L.; Harte, Federico Miguel; Campagna, Shawn R.; De Veth, Michael J.

In: PloS one, Vol. 9, No. 8, e103412, 26.08.2014.

Research output: Contribution to journalArticle

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AU - Middleton, Jesse L.

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AU - De Veth, Michael J.

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AB - Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in early, mid and late lactation and analyzed for acetylcholine, free choline, betaine, glycerophosphocholine, lysophosphatidylcholine, phosphatidylcholine, phosphocholine and sphingomyelin using hydrophilic interaction liquid chromatography-tandem mass spectrometry, and quantified using stable isotope-labeled internal standards. Total choline concentration in plasma, which was almost entirely phosphatidylcholine, increased 10-times from early to late lactation (1305 to 13,535 μmol/L). In milk, phosphocholine was the main metabolite in early lactation (492 μmol/L), which is a similar concentration to that found in human milk, however, phosphocholine concentration decreased exponentially through lactation to 43 μmol/L in late lactation. In contrast, phosphatidylcholine was the main metabolite in mid and late lactation (188 μmol/L and 659 μmol/L, respectively), with the increase through lactation positively correlated with phosphatidylcholine in plasma (R2 = 0.78). Unlike previously reported with human milk we found no correlation between plasma free choline concentration and milk choline metabolites. The changes in pattern of phosphocholine and phosphatidylcholine in milk through lactation observed in the bovine suggests that it is possible to manufacture infant formula that more closely matches these metabolites profile in human milk.

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