Chromatic two-photon excitation fluorescence imaging

Q. Xu, K. Shi, Shizhuo Yin, Zhiwen Liu

Research output: Contribution to journalArticle

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Abstract

We report on a chromatic axial scanning method for two-photon excitation fluorescence imaging. Effective axial scanning is achieved by incorporating a Fresnel lens in the system, which has large chromatic aberration and can therefore focus the excitation beam to different axial positions depending on its wavelength. We experimentally demonstrated this technique and used it to image the cross-section of fluorescent microspheres.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalJournal of Microscopy
Volume235
Issue number1
DOIs
StatePublished - Jul 1 2009

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All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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