Chromatin architectures at fission yeast transcriptional promoters and replication origins

Robert M. Givens, William K.M. Lai, Jason M. Rizzo, Jonathan E. Bard, Piotr A. Mieczkowski, Janet Leatherwood, Joel A. Huberman, Michael J. Buck

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Abstract

We have used micrococcal nuclease (MNase) digestion followed by deep sequencing in order to obtain a higher resolution map than previously available of nucleosome positions in the fission yeast, Schizosaccharomyces pombe. Our data confirm an unusually short average nucleosome repeat length, ∼152 bp, in fission yeast and that transcriptional start sites (TSSs) are associated with nucleosomedepleted regions (NDRs), ordered nucleosome arrays downstream and less regularly spaced upstreamnucleosomes. In addition, we found enrichments for associated function in four of eight groups of genes clustered according to chromatin configurations near TSSs. At replication origins, our data revealed asymmetric localization of pre-replication complex (pre-RC) proteins within large NDRs-a feature that is conserved in fission and budding yeast and is therefore likely to be conserved in other eukaryotic organisms.

Original languageEnglish (US)
Pages (from-to)7176-7189
Number of pages14
JournalNucleic acids research
Volume40
Issue number15
DOIs
Publication statusPublished - Aug 1 2012

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All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Givens, R. M., Lai, W. K. M., Rizzo, J. M., Bard, J. E., Mieczkowski, P. A., Leatherwood, J., ... Buck, M. J. (2012). Chromatin architectures at fission yeast transcriptional promoters and replication origins. Nucleic acids research, 40(15), 7176-7189. https://doi.org/10.1093/nar/gks351