Chromatin structure and nuclear remodeling

Kristen M. Johansen, Jørgen Johansen, Ye Jin, Diana L. Walker, Dong Wang, Yanming Wang

Research output: Contribution to journalReview article

7 Scopus citations

Abstract

Nuclear architecture is remodeled during interphase in response to changes in gene activity as well as to changing structural and functional requirements during cell division. Using the monoclonal antibody mAb2A, we have identified two proteins that appear to play important roles in these processes: JIL-1 is a tandem serine-threonine kinase implicated in the regulation of chromatin structure, whereas Skeletor is a novel protein participating in structural nuclear remodeling during the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost twofold on the hypertranscribed male X chromosome compared with autosomes. We propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure. The other mAb2A antigen, Skeletor, is distributed in a nuclear meshwork pattern that can be observed in stereo pair images to reorganize during the cell cycle to form a spindle-like structure at prometaphase that is distinct from the microtubule spindle apparatus. Taking advantage of the powerful molecular and genetic approaches offered in Drosophila, the study of these two proteins promises to yield new insight into what defines nuclear architecture at the molecular level and how its remodeling is regulated.

Original languageEnglish (US)
Pages (from-to)267-277
Number of pages11
JournalCritical Reviews in Eukaryotic Gene Expression
Volume9
Issue number3-4
DOIs
StatePublished - Jan 1 1999

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Johansen, K. M., Johansen, J., Jin, Y., Walker, D. L., Wang, D., & Wang, Y. (1999). Chromatin structure and nuclear remodeling. Critical Reviews in Eukaryotic Gene Expression, 9(3-4), 267-277. https://doi.org/10.1615/CritRevEukarGeneExpr.v9.i3-4.110