Chromatin structure dynamics during the mitosis-to-G1 phase transition

Haoyue Zhang, Daniel J. Emerson, Thomas G. Gilgenast, Katelyn R. Titus, Yemin Lan, Peng Huang, Di Zhang, Hongxin Wang, Cheryl A. Keller, Belinda Giardine, Ross C. Hardison, Jennifer E. Phillips-Cremins, Gerd A. Blobel

Research output: Contribution to journalArticle

Abstract

Features of higher-order chromatin organization—such as A/B compartments, topologically associating domains and chromatin loops—are temporarily disrupted during mitosis1,2. Because these structures are thought to influence gene regulation, it is important to understand how they are re-established after mitosis. Here we examine the dynamics of chromosome reorganization by Hi-C after mitosis in highly purified, synchronous mouse erythroid cell populations. We observed rapid establishment of A/B compartments, followed by their gradual intensification and expansion. Contact domains form from the ‘bottom up’—smaller subTADs are formed initially, followed by convergence into multi-domain TAD structures. CTCF is partially retained on mitotic chromosomes and immediately resumes full binding in ana/telophase. By contrast, cohesin is completely evicted from mitotic chromosomes and regains focal binding at a slower rate. The formation of CTCF/cohesin co-anchored structural loops follows the kinetics of cohesin positioning. Stripe-shaped contact patterns—anchored by CTCF—grow in length, which is consistent with a loop-extrusion process after mitosis. Interactions between cis-regulatory elements can form rapidly, with rates exceeding those of CTCF/cohesin-anchored contacts. Notably, we identified a group of rapidly emerging transient contacts between cis-regulatory elements in ana/telophase that are dissolved upon G1 entry, co-incident with the establishment of inner boundaries or nearby interfering chromatin loops. We also describe the relationship between transcription reactivation and architectural features. Our findings indicate that distinct but mutually influential forces drive post-mitotic chromatin reconfiguration.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalNature
Volume576
Issue number7785
DOIs
StatePublished - Dec 5 2019

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Phase Transition
G1 Phase
Mitosis
Chromatin
Telophase
Chromosomes
Erythroid Cells
cohesins
Population
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Zhang, H., Emerson, D. J., Gilgenast, T. G., Titus, K. R., Lan, Y., Huang, P., ... Blobel, G. A. (2019). Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature, 576(7785), 158-162. https://doi.org/10.1038/s41586-019-1778-y
Zhang, Haoyue ; Emerson, Daniel J. ; Gilgenast, Thomas G. ; Titus, Katelyn R. ; Lan, Yemin ; Huang, Peng ; Zhang, Di ; Wang, Hongxin ; Keller, Cheryl A. ; Giardine, Belinda ; Hardison, Ross C. ; Phillips-Cremins, Jennifer E. ; Blobel, Gerd A. / Chromatin structure dynamics during the mitosis-to-G1 phase transition. In: Nature. 2019 ; Vol. 576, No. 7785. pp. 158-162.
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Zhang, H, Emerson, DJ, Gilgenast, TG, Titus, KR, Lan, Y, Huang, P, Zhang, D, Wang, H, Keller, CA, Giardine, B, Hardison, RC, Phillips-Cremins, JE & Blobel, GA 2019, 'Chromatin structure dynamics during the mitosis-to-G1 phase transition', Nature, vol. 576, no. 7785, pp. 158-162. https://doi.org/10.1038/s41586-019-1778-y

Chromatin structure dynamics during the mitosis-to-G1 phase transition. / Zhang, Haoyue; Emerson, Daniel J.; Gilgenast, Thomas G.; Titus, Katelyn R.; Lan, Yemin; Huang, Peng; Zhang, Di; Wang, Hongxin; Keller, Cheryl A.; Giardine, Belinda; Hardison, Ross C.; Phillips-Cremins, Jennifer E.; Blobel, Gerd A.

In: Nature, Vol. 576, No. 7785, 05.12.2019, p. 158-162.

Research output: Contribution to journalArticle

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AU - Zhang, Haoyue

AU - Emerson, Daniel J.

AU - Gilgenast, Thomas G.

AU - Titus, Katelyn R.

AU - Lan, Yemin

AU - Huang, Peng

AU - Zhang, Di

AU - Wang, Hongxin

AU - Keller, Cheryl A.

AU - Giardine, Belinda

AU - Hardison, Ross C.

AU - Phillips-Cremins, Jennifer E.

AU - Blobel, Gerd A.

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Zhang H, Emerson DJ, Gilgenast TG, Titus KR, Lan Y, Huang P et al. Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature. 2019 Dec 5;576(7785):158-162. https://doi.org/10.1038/s41586-019-1778-y