Chromatographic conditions for separation phosphates of p-labeled phosphates of major polynuclear aromatic hydrocarbon-deoxyribonucleoside adducts

Shantu Amin, Bijaya Misra, Dhimant Desai, Kieth Keith, Stephen S. Hecht

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24 Scopus citations

Abstract

The 3'-monophosphates of the major deoxyr ibonucleoside adducts of five representative polynuclear aromatic hydro carbons (PAH)-benzo[a]pyrene, benz[a]anthracene, chrysene, 5-methylchrysene and 6-methylchrysene-were prepared by reaction with DNA of the appropriate bay-region diol epoxides followed by enzymatic hydrolysis and HPLC purification. These standards were converted to 3', [32p]-5'-bisphosphates as in the Randerath 32P-postlabeling assay. TLC and HPLC systems for their separation are described. HPLC conditions for separation of the corresponding 3'-and 5'monophosphates are also reported. These data will be useful for determining whether unknown DNA adducts detected by the 32P-postlabeling assay may have resulted from reactions of DNA with PAH diol epoxides.

Original languageEnglish (US)
Pages (from-to)1971-1974
Number of pages4
JournalCarcinogenesis
Volume10
Issue number10
DOIs
StatePublished - Oct 1 1989

All Science Journal Classification (ASJC) codes

  • Cancer Research

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