Chromatographic conditions for separation phosphates of p-labeled phosphates of major polynuclear aromatic hydrocarbon-deoxyribonucleoside adducts

Shantu Amin, Bijaya Misra, Dhimant Desai, Kieth Keith, Stephen S. Hecht

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The 3'-monophosphates of the major deoxyr ibonucleoside adducts of five representative polynuclear aromatic hydro carbons (PAH)-benzo[a]pyrene, benz[a]anthracene, chrysene, 5-methylchrysene and 6-methylchrysene-were prepared by reaction with DNA of the appropriate bay-region diol epoxides followed by enzymatic hydrolysis and HPLC purification. These standards were converted to 3', [32p]-5'-bisphosphates as in the Randerath 32P-postlabeling assay. TLC and HPLC systems for their separation are described. HPLC conditions for separation of the corresponding 3'-and 5'monophosphates are also reported. These data will be useful for determining whether unknown DNA adducts detected by the 32P-postlabeling assay may have resulted from reactions of DNA with PAH diol epoxides.

Original languageEnglish (US)
Pages (from-to)1971-1974
Number of pages4
JournalCarcinogenesis
Volume10
Issue number10
DOIs
StatePublished - Oct 1 1989

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Deoxyribonucleosides
Aromatic hydrocarbons
High-performance Liquid Chromatography
Polycyclic Aromatic Hydrocarbons
Hydrocarbons
Phosphate
Assays
Phosphates
DNA
Epoxy Compounds
High Pressure Liquid Chromatography
Carbon
Enzymatic hydrolysis
Anthracene
Pyrene
Purification
DNA Adducts
Benzo(a)pyrene
Hydrolysis
Unknown

All Science Journal Classification (ASJC) codes

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

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abstract = "The 3'-monophosphates of the major deoxyr ibonucleoside adducts of five representative polynuclear aromatic hydro carbons (PAH)-benzo[a]pyrene, benz[a]anthracene, chrysene, 5-methylchrysene and 6-methylchrysene-were prepared by reaction with DNA of the appropriate bay-region diol epoxides followed by enzymatic hydrolysis and HPLC purification. These standards were converted to 3', [32p]-5'-bisphosphates as in the Randerath 32P-postlabeling assay. TLC and HPLC systems for their separation are described. HPLC conditions for separation of the corresponding 3'-and 5'monophosphates are also reported. These data will be useful for determining whether unknown DNA adducts detected by the 32P-postlabeling assay may have resulted from reactions of DNA with PAH diol epoxides.",
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Chromatographic conditions for separation phosphates of p-labeled phosphates of major polynuclear aromatic hydrocarbon-deoxyribonucleoside adducts. / Amin, Shantu; Misra, Bijaya; Desai, Dhimant; Keith, Kieth; Hecht, Stephen S.

In: Carcinogenesis, Vol. 10, No. 10, 01.10.1989, p. 1971-1974.

Research output: Contribution to journalArticle

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T1 - Chromatographic conditions for separation phosphates of p-labeled phosphates of major polynuclear aromatic hydrocarbon-deoxyribonucleoside adducts

AU - Amin, Shantu

AU - Misra, Bijaya

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AU - Hecht, Stephen S.

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