Chromosomal transformation in the cyanobacterium Agmenellum quadruplicatum

E. Essich, E. Stevens, R. D. Porter

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Chromosomal transformation of Agmenellum quadruplicatum PR-6 (=Synechococcus sp. strain 7002) was characterized for phenotypic expression, for exposure time to DNA, and for dependence on DNA concentration with regard to Rif(r) donor DNA. Exponentially growing cells of PR-6 were competent for chromosomal transformation. Competence decreased in cells in the stationary phase of growth or in cells deprived of a nitrogen source. Dark incubation of cells before exposure to donor DNA also decreased competence. Homologous Rif(r) and Str(r) DNA and heterologous Escherichia coli W3110 DNA were used in DNA-DNA competition studies, which clearly showed that DNA binding by PR-6 was nonspecific. DNA binding and uptake by PR-6 exhibited single-hit kinetics. Single-stranded DNA failed to transform competent cells of PR-6, and uptake reactions during the transformation of PR-6. A significant improvement in transformation frequency was achieved by increasing the nitrate content of the culture medium and by lowering the temperature at which cells were exposed to donor DNA from 39°C (the optimal temperature for growth) to 30°C.

Original languageEnglish (US)
Pages (from-to)1916-1922
Number of pages7
JournalJournal of bacteriology
Volume172
Issue number4
DOIs
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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