Chronic optical access through a polished and reinforced thinned skull

Patrick James Drew, Andy Y. Shih, Jonathan D. Driscoll, Per Magne Knutsen, Pablo Blinder, Dimitrios Davalos, Katerina Akassoglou, Philbert S. Tsai, David Kleinfeld

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.

Original languageEnglish (US)
Pages (from-to)981-984
Number of pages4
JournalNature methods
Volume7
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

Photosensitizing Agents
Skull
Brain
Blood
Lighting
Optogenetics
Dendritic Spines
Microglia
Encephalitis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Drew, P. J., Shih, A. Y., Driscoll, J. D., Knutsen, P. M., Blinder, P., Davalos, D., ... Kleinfeld, D. (2010). Chronic optical access through a polished and reinforced thinned skull. Nature methods, 7(12), 981-984. https://doi.org/10.1038/nmeth.1530
Drew, Patrick James ; Shih, Andy Y. ; Driscoll, Jonathan D. ; Knutsen, Per Magne ; Blinder, Pablo ; Davalos, Dimitrios ; Akassoglou, Katerina ; Tsai, Philbert S. ; Kleinfeld, David. / Chronic optical access through a polished and reinforced thinned skull. In: Nature methods. 2010 ; Vol. 7, No. 12. pp. 981-984.
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Drew, PJ, Shih, AY, Driscoll, JD, Knutsen, PM, Blinder, P, Davalos, D, Akassoglou, K, Tsai, PS & Kleinfeld, D 2010, 'Chronic optical access through a polished and reinforced thinned skull', Nature methods, vol. 7, no. 12, pp. 981-984. https://doi.org/10.1038/nmeth.1530

Chronic optical access through a polished and reinforced thinned skull. / Drew, Patrick James; Shih, Andy Y.; Driscoll, Jonathan D.; Knutsen, Per Magne; Blinder, Pablo; Davalos, Dimitrios; Akassoglou, Katerina; Tsai, Philbert S.; Kleinfeld, David.

In: Nature methods, Vol. 7, No. 12, 01.12.2010, p. 981-984.

Research output: Contribution to journalArticle

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Drew PJ, Shih AY, Driscoll JD, Knutsen PM, Blinder P, Davalos D et al. Chronic optical access through a polished and reinforced thinned skull. Nature methods. 2010 Dec 1;7(12):981-984. https://doi.org/10.1038/nmeth.1530