Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival

Phillip J. Albrecht, John P. Dahl, Olivia K. Stoltzfus, Robert Levenson, Steven W. Levison

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

At focal CNS injury sites, several cytokines accumulate, including ciliary neurotrophic factor (CNTF) and interleukin-1β (IL-1β). Additionally, the CNTF alpha receptor is induced on astrocytes, establishing an autocrine/paracrine loop. How astrocyte function is altered as a result of CNTF stimulation remains incompletely characterized. Here, we demonstrate that direct injection of CNTF into the spinal cord increases GFAP expression and astroglial size and that primary cultures of spinal cord astrocytes treated with CNTF, IL-1β, or leukemia inhibitory factor exhibit nuclear hypertrophy comparable to that observed in vivo. Using a coculture bioassay, we further demonstrate that CNTF treatment of astrocytes increases their ability to support ChAT+ ventral spinal cord neurons (presumably motor neurons) more than twofold compared with untreated astrocytes. Also, the complexity of neurites was significantly increased in neurons cultured with CNTF-treated astrocytes compared with untreated astrocytes. RT-PCR analysis demonstrated that CNTF increased levels of FGF-2 and nerve growth factor (NGF) mRNA and that IL-1β increased NGF and hepatocyte growth factor mRNA levels. Furthermore, both CNTF and IL-1β stimulated the release of FGF-2 from cultured spinal cord astrocytes. These findings demonstrate that cytokine-activated astrocytes better support CNS neuron survival via the production of neurotrophic molecules. We also show that CNTF synergizes with FGF-2, but not epidermal growth factor, to promote DNA synthesis in spinal cord astrocyte cultures. The significance of these findings is discussed by presenting a new model depicting the sequential activation of astrocytes by cytokines and growth factors in the context of CNS injury and repair.

Original languageEnglish (US)
Pages (from-to)46-62
Number of pages17
JournalExperimental Neurology
Volume173
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Ciliary Neurotrophic Factor
Motor Neurons
Fibroblast Growth Factor 2
Astrocytes
Spinal Cord
Interleukin-1
Nerve Growth Factor
Cytokines
Neurons
Ciliary Neurotrophic Factor Receptor
Leukemia Inhibitory Factor
Messenger RNA
Hepatocyte Growth Factor
Wounds and Injuries
Neurites
Coculture Techniques
Epidermal Growth Factor
Biological Assay
Hypertrophy
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience

Cite this

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title = "Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival",
abstract = "At focal CNS injury sites, several cytokines accumulate, including ciliary neurotrophic factor (CNTF) and interleukin-1β (IL-1β). Additionally, the CNTF alpha receptor is induced on astrocytes, establishing an autocrine/paracrine loop. How astrocyte function is altered as a result of CNTF stimulation remains incompletely characterized. Here, we demonstrate that direct injection of CNTF into the spinal cord increases GFAP expression and astroglial size and that primary cultures of spinal cord astrocytes treated with CNTF, IL-1β, or leukemia inhibitory factor exhibit nuclear hypertrophy comparable to that observed in vivo. Using a coculture bioassay, we further demonstrate that CNTF treatment of astrocytes increases their ability to support ChAT+ ventral spinal cord neurons (presumably motor neurons) more than twofold compared with untreated astrocytes. Also, the complexity of neurites was significantly increased in neurons cultured with CNTF-treated astrocytes compared with untreated astrocytes. RT-PCR analysis demonstrated that CNTF increased levels of FGF-2 and nerve growth factor (NGF) mRNA and that IL-1β increased NGF and hepatocyte growth factor mRNA levels. Furthermore, both CNTF and IL-1β stimulated the release of FGF-2 from cultured spinal cord astrocytes. These findings demonstrate that cytokine-activated astrocytes better support CNS neuron survival via the production of neurotrophic molecules. We also show that CNTF synergizes with FGF-2, but not epidermal growth factor, to promote DNA synthesis in spinal cord astrocyte cultures. The significance of these findings is discussed by presenting a new model depicting the sequential activation of astrocytes by cytokines and growth factors in the context of CNS injury and repair.",
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Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival. / Albrecht, Phillip J.; Dahl, John P.; Stoltzfus, Olivia K.; Levenson, Robert; Levison, Steven W.

In: Experimental Neurology, Vol. 173, No. 1, 01.01.2002, p. 46-62.

Research output: Contribution to journalArticle

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T1 - Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival

AU - Albrecht, Phillip J.

AU - Dahl, John P.

AU - Stoltzfus, Olivia K.

AU - Levenson, Robert

AU - Levison, Steven W.

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