Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia

Jiaojiao Hu, Qi Han, Yan Gu, Jinlong Ma, Mary McGrath, Fengchang Qiao, Baoan Chen, Chunhua Song, Zheng Ge

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aim: The roles of circular RNA PVT1 (circPVT1) are explored in the patients with acute lymphoblastic leukemia (ALL). Methods: The circPVT1 level was detected by qRT-PCR and western blot. The apoptotic cells were examined by the annexin V assay in lentiviral shRNA knockdown cells. Results: circPVT1 was highly expressed in ALL compared with normal bone marrow samples. circPVT1 expression was also significantly higher in ALL cell lines. circPVT1 knockdown inhibited cell proliferation and induced cell apoptosis through suppression of its neighbor gene c-Myc, and antiapoptotic Bcl-2 protein expression. Conclusion: circPVT1 is upregulated in ALL. Silencing circPVT1 results in cell growth arrest and apoptosis of the cells. Our results also suggested a therapeutic potential of targeting circPVT1 in ALL.

Original languageEnglish (US)
Pages (from-to)723-732
Number of pages10
JournalEpigenomics
Volume10
Issue number6
DOIs
StatePublished - Jun 2018

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
Apoptosis
myc Genes
circular RNA
Annexin A5
Small Interfering RNA
Bone Marrow
Western Blotting
Cell Proliferation
Cell Line
Polymerase Chain Reaction
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cancer Research

Cite this

Hu, J., Han, Q., Gu, Y., Ma, J., McGrath, M., Qiao, F., ... Ge, Z. (2018). Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia. Epigenomics, 10(6), 723-732. https://doi.org/10.2217/epi-2017-0142
Hu, Jiaojiao ; Han, Qi ; Gu, Yan ; Ma, Jinlong ; McGrath, Mary ; Qiao, Fengchang ; Chen, Baoan ; Song, Chunhua ; Ge, Zheng. / Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia. In: Epigenomics. 2018 ; Vol. 10, No. 6. pp. 723-732.
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Hu, J, Han, Q, Gu, Y, Ma, J, McGrath, M, Qiao, F, Chen, B, Song, C & Ge, Z 2018, 'Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia', Epigenomics, vol. 10, no. 6, pp. 723-732. https://doi.org/10.2217/epi-2017-0142

Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia. / Hu, Jiaojiao; Han, Qi; Gu, Yan; Ma, Jinlong; McGrath, Mary; Qiao, Fengchang; Chen, Baoan; Song, Chunhua; Ge, Zheng.

In: Epigenomics, Vol. 10, No. 6, 06.2018, p. 723-732.

Research output: Contribution to journalArticle

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AU - Gu, Yan

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