Circulating human immunodeficiency virus (HIV) p24 antigen-positive lymphocytes: A flow cytometric measure of HIV infection

Betsy M. Ohlsson-Wilhelm, Joan M. Cory, Harold A. Kessler, Elaine E. Eyster, Fred Rapp, Alan Landay

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    Abstract

    Asymptomatic individuals seropositive for human immunodeficiency virus (HIV) progress in a heterogeneous fashion toward AIDS. To facilitate monitoring of disease progression and response to therapy, a rapid, new flow cytometric assay (FCA) lymphocyte p24-FCA, has been devised to quantify peripheral blood lymphocytes expressing cell-associated HIV-l p24 antigen. Results from 55 asymptomatic, HIV-1-seropositive, serum p24 antigen-negative individuals ranged from undetectable (<0.1%) to 13.6% p24+ lymphocytes (mean, 2.0%). Mean values for three other groups studied were 0.1% for seronegative, viral culture-negative laboratory workers (n = 24); 4.2% for untreated patients with AIDS (n = 16); and 0.3% for AIDS patients receiving zidovudine (n = 11). Lymphocyte p24-FCA values were inversel)Helated to the number of days to positive viral cultures and to levels of CD4+ lymphocytes. The ratio of p24+ lymphocytes to CD4+ lymphocytes may reflect the fraction of infected CD4+ lymphocytes. Lymphocyte p24-FCA determination may provide a method for monitoring response to antiretroviral therapy regardless of serum p24 antigen status.

    Original languageEnglish (US)
    Pages (from-to)1018-1024
    Number of pages7
    JournalJournal of Infectious Diseases
    Volume162
    Issue number5
    DOIs
    StatePublished - Nov 1990

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    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Infectious Diseases

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