Class I gene contraction within the HLA-A subregion of the human MHC

Charles P. Venditti, Michael J. Chorney

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Individuals expressing either the HLA-A24 or the HLA-A23 histocompatibility antigens have been found to possess an HLA-A class I subregion approximately 50 kb smaller in size than those studied from individuals expressing other HLA-A haplotypes. This originally manifested itself as a haplotype-associated size variation in the NotI and MluI megabase fragments observed on pulsed-field electrophoresis gels after blotting and probing with HLA-A subregion-specific genomic probes. The contracted region falls between the HLA-A and the HLA-G class I genes and specifically includes the novel HLA-A-related pseudogene, HLA-H, as well as the adjacent deteriorated class I pseudogene, 7.0 p. The intactness of locus D6S128, defined by probe pMC6.7 located telomeric to the HLA-H gene, demonstrates that the distal rearrangement point falls within a 20-kb stretch of DNA separating HLA-H from pMC6.7. This extends a previous report regarding variation in class I gene number within the human major histocompatibility complex and precisely localizes the genomic residence of sequences that may define a recombination hot spot. Because the size variation maps to a recombinogenic area, its characterization may ultimately reveal important biological information relevant to the events that shaped the organization of the human HLA class I multigene family.

Original languageEnglish (US)
Pages (from-to)1003-1009
Number of pages7
JournalGenomics
Volume14
Issue number4
DOIs
StatePublished - Dec 1992

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MHC Class I Genes
HLA-A Antigens
Pseudogenes
Haplotypes
HLA-A24 Antigen
HLA-G Antigens
Histocompatibility Antigens
Pulsed Field Gel Electrophoresis
Multigene Family
Major Histocompatibility Complex
Genetic Recombination
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Venditti, Charles P. ; Chorney, Michael J. / Class I gene contraction within the HLA-A subregion of the human MHC. In: Genomics. 1992 ; Vol. 14, No. 4. pp. 1003-1009.
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Class I gene contraction within the HLA-A subregion of the human MHC. / Venditti, Charles P.; Chorney, Michael J.

In: Genomics, Vol. 14, No. 4, 12.1992, p. 1003-1009.

Research output: Contribution to journalArticle

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