Clinical translation of a novel microfilter technology: Capture, characterization and culture of circulating tumor cells

Anthony Williams, Siddarth Rawal, Zheng Ao, Jorge Torres-Munoz, Marija Balic, Mingda Zhou, Siyang Zheng, Yu Chong Tai, Richard J. Cote, Ram Datar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

The most important determinant of prognosis and management of cancer is the presence or absence of metastasis [1]. The road to metastasis involves tumor cells to become detached from the primary tumor and travel in the blood to distant sites, causing secondary tumors. These tumor cells traveling in blood are termed Circulating tumor cells (CTC). Capture of CTC from whole blood has been a challenging feat. The fact that these CTC are few in number, to effectively and efficiently isolate them from whole blood can be thought of as looking for a needle in a haystack. Our microfilter technology exploits the use of size based capture of the larger CTC from the smaller white blood cells and components of whole blood. The effective capture potential of the microfilter platform has driven the area of CTC analysis into a new age of research in the field of cancer. The ability to finally analyze CTC at a molecular level, leads to a deeper understanding of metastatic process, while providing an opportunity to evaluate, monitor and manage treatment options as well as the adherent possibility of having an "on-chip" drug sensitivity assay for focused treatment options. We have demonstrated through clinical trials the ability to effectively identify, enumerate and characterize CTC based on immunfluorescence and FISH assays and provide a companion endpoint for monitoring and evaluating treatment management. Our work on viable CTC capture has resulted in successfully capturing and culturing CTC from blood in mouse models that have been inoculated with breast cancer cell lines to form primary and secondary metastatic cancer sites. The future potential within the microfilter technology to capture viable CTC for culture, will catapult therapeutic interventions to a new level of personalized medicine in cancer management.

Original languageEnglish (US)
Title of host publicationIEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies
Subtitle of host publicationSynergy Towards Better Global Healthcare, PHT 2013
Pages220-223
Number of pages4
DOIs
StatePublished - Mar 8 2013
Event1st IEEE-EMBS Conference on Point-of-Care Healthcare Technologies, PHT 2013 - Bangalore, India
Duration: Jan 16 2013Jan 18 2013

Publication series

NameIEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013

Other

Other1st IEEE-EMBS Conference on Point-of-Care Healthcare Technologies, PHT 2013
CountryIndia
CityBangalore
Period1/16/131/18/13

Fingerprint

Circulating Neoplastic Cells
Cell culture
Tumors
Cells
Technology
Blood
Neoplasms
Neoplasm Metastasis
Precision Medicine
Assays
Cellular Structures
Therapeutics
Leukocytes
Cell Culture Techniques
Clinical Trials
Breast Neoplasms

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Health Informatics
  • Health Information Management

Cite this

Williams, A., Rawal, S., Ao, Z., Torres-Munoz, J., Balic, M., Zhou, M., ... Datar, R. (2013). Clinical translation of a novel microfilter technology: Capture, characterization and culture of circulating tumor cells. In IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013 (pp. 220-223). [6461324] (IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013). https://doi.org/10.1109/PHT.2013.6461324
Williams, Anthony ; Rawal, Siddarth ; Ao, Zheng ; Torres-Munoz, Jorge ; Balic, Marija ; Zhou, Mingda ; Zheng, Siyang ; Tai, Yu Chong ; Cote, Richard J. ; Datar, Ram. / Clinical translation of a novel microfilter technology : Capture, characterization and culture of circulating tumor cells. IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013. 2013. pp. 220-223 (IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013).
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author = "Anthony Williams and Siddarth Rawal and Zheng Ao and Jorge Torres-Munoz and Marija Balic and Mingda Zhou and Siyang Zheng and Tai, {Yu Chong} and Cote, {Richard J.} and Ram Datar",
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Williams, A, Rawal, S, Ao, Z, Torres-Munoz, J, Balic, M, Zhou, M, Zheng, S, Tai, YC, Cote, RJ & Datar, R 2013, Clinical translation of a novel microfilter technology: Capture, characterization and culture of circulating tumor cells. in IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013., 6461324, IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013, pp. 220-223, 1st IEEE-EMBS Conference on Point-of-Care Healthcare Technologies, PHT 2013, Bangalore, India, 1/16/13. https://doi.org/10.1109/PHT.2013.6461324

Clinical translation of a novel microfilter technology : Capture, characterization and culture of circulating tumor cells. / Williams, Anthony; Rawal, Siddarth; Ao, Zheng; Torres-Munoz, Jorge; Balic, Marija; Zhou, Mingda; Zheng, Siyang; Tai, Yu Chong; Cote, Richard J.; Datar, Ram.

IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013. 2013. p. 220-223 6461324 (IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Williams, Anthony

AU - Rawal, Siddarth

AU - Ao, Zheng

AU - Torres-Munoz, Jorge

AU - Balic, Marija

AU - Zhou, Mingda

AU - Zheng, Siyang

AU - Tai, Yu Chong

AU - Cote, Richard J.

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Williams A, Rawal S, Ao Z, Torres-Munoz J, Balic M, Zhou M et al. Clinical translation of a novel microfilter technology: Capture, characterization and culture of circulating tumor cells. In IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013. 2013. p. 220-223. 6461324. (IEEE EMBS Special Topic Conference on Point-of-Care (POC) Healthcare Technologies: Synergy Towards Better Global Healthcare, PHT 2013). https://doi.org/10.1109/PHT.2013.6461324