Lysophosphatidic acid (LPA) which is originally known as an intermediate of de novo phospholipid synthesis, has recently been recognized as an intercellular phospholipid messenger with various biological activities. There have been accumulating evidence that LPA functions through one or more G protein-coupled receptors. Recently, two putative LPA receptors were cloned independently. Vzg (ventricular zone gene) -1 was cloned from mouse cerebral cortex-derived cell line, and PSP24 was cloned from Xenopus oocytes. From their predicted amino acid sequences, these two receptors contained seven membrane-spanning domains. But they share little sequence homology. To clarify the characteristics of mammalian LPA receptor, we isolated mouse PSP24 (mPSP24) gene from genomic library. It contains a putative open reading frame corresponding to the full length Xenopus cDNA, and they share a high sequence homology. The analysis of mPSP24 gene expression in multiple tissues revealed that it was highly expressed in brain. mPSP24 mRNA localization in brain was also shown by using in situ hybridization technique. The electrophysiological analysis with voltage-clamp technique using Xenopus oocytes showed that mPSP24-overexpression potentiated LPA-induced Cl~ current. We concluded that mPSP24 is an LPA receptor expressed in nervous system. Ref. Guo Z. et al. ( 1996) Molecular cloning of a high-affinity receptor for the growth factor-like lipid mediator lysophosphatidic acid from Xenopus oocytes. Proc. Natl. Acad. Sei. USA 93: 14567-72.
|Original language||English (US)|
|State||Published - 1998|
All Science Journal Classification (ASJC) codes
- Molecular Biology