Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases

Douglas Stairs, Heather Perry Gardner, Seung I. Ha, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Lewis A. Chodosh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Protein kinases frequently play key roles in the normal regulation of growth and development in eukaryotic organisms. As a consequence, aberrant expression or mutations in this family of molecules frequently result in transformation. Previously, we have conducted a screen to identify protein kinases that are expressed in the mouse during mammary gland development and in breast cancer cell lines. We now describe the molecular cloning, characterization and expression of Krct, a novel serine/threonine protein kinase unrelated to previously defined families of protein kinases. At the mRNA level, Krct is widely expressed throughout murine development and in adult tissues. Despite its ubiquitous expression, Krct is expressed preferentially within specific cellular compartments in multiple tissues, in particular within the testis and gastrointestinal tract. At the amino acid level, Krct is most closely related to four previously undescribed kinases in Saccharomyces cerevisiae. Arabidopsis thaliana and Caenorhabditis elegans. Together, these kinases appear to define a novel subfamily of serine/threonine protein kinases, Krct possesses an unusually long 5'-untranslated region containing multiple upstream initiation codons and, in this regard, is similar to many proto-oncogenes that regulate normal growth and differentiation. In addition, Krct is located on mouse chromosome 11 closely linked to the epidermal growth factor receptor and, therefore, is likely to be co-amplified in a variety of human tumors.

Original languageEnglish (US)
Pages (from-to)2157-2166
Number of pages10
JournalHuman Molecular Genetics
Volume7
Issue number13
StatePublished - Dec 1 1998

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Protein-Serine-Threonine Kinases
Protein Kinases
Organism Cloning
Phosphotransferases
Chromosomes, Human, Pair 11
Initiator Codon
Proto-Oncogenes
5' Untranslated Regions
Caenorhabditis elegans
Molecular Cloning
Human Mammary Glands
Growth and Development
Epidermal Growth Factor Receptor
Arabidopsis
Saccharomyces cerevisiae
Gastrointestinal Tract
Testis
Breast Neoplasms
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Stairs, D., Gardner, H. P., Ha, S. I., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., & Chodosh, L. A. (1998). Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases. Human Molecular Genetics, 7(13), 2157-2166.
Stairs, Douglas ; Gardner, Heather Perry ; Ha, Seung I. ; Copeland, Neal G. ; Gilbert, Debra J. ; Jenkins, Nancy A. ; Chodosh, Lewis A. / Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases. In: Human Molecular Genetics. 1998 ; Vol. 7, No. 13. pp. 2157-2166.
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Stairs, D, Gardner, HP, Ha, SI, Copeland, NG, Gilbert, DJ, Jenkins, NA & Chodosh, LA 1998, 'Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases', Human Molecular Genetics, vol. 7, no. 13, pp. 2157-2166.

Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases. / Stairs, Douglas; Gardner, Heather Perry; Ha, Seung I.; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Chodosh, Lewis A.

In: Human Molecular Genetics, Vol. 7, No. 13, 01.12.1998, p. 2157-2166.

Research output: Contribution to journalArticle

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Stairs D, Gardner HP, Ha SI, Copeland NG, Gilbert DJ, Jenkins NA et al. Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases. Human Molecular Genetics. 1998 Dec 1;7(13):2157-2166.