Cloning and characterizing of the ovine MX1 gene promoter/enhancer region

A. M. Assiri, Troy Ott

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ovine MX1 (MX1) is expressed in the uterus during the estrous cycle and is strongly up-regulated during early pregnancy in the uterus and peripheral blood leukocytes. In this study we cloned the MX1 gene promoter/enhancer, and tested its response to interferon tau (IFN-tau). To address the role of IFN tau in regulating MX1 expression, serial deletion mutants were prepared along with a clone that contained a full-length promoter including the two proximal ISREs but lacking an intronic ISRE site. Promoter deletions showed the two proximal ISRE sites, but not the intronic ISRE site, were required for maximal response to IFN tau. Interestingly, MX1 promoter deletion mutants revealed the presence of distal positive (-920 to -715) and negative (-715 to -437) regulatory regions. Identifying positive and negative regulatory regions in MX1 promoter will help define the complex regulation of MX1 during early pregnancy in ruminants.

Original languageEnglish (US)
Pages (from-to)847-857
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume31
Issue number8
DOIs
StatePublished - Apr 13 2007

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Nucleic Acid Regulatory Sequences
Genetic Promoter Regions
Uterus
Organism Cloning
Sheep
Pregnancy
Estrous Cycle
Ruminants
Genes
Leukocytes
Clone Cells
MX(1) complex
trophoblastin

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

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abstract = "Ovine MX1 (MX1) is expressed in the uterus during the estrous cycle and is strongly up-regulated during early pregnancy in the uterus and peripheral blood leukocytes. In this study we cloned the MX1 gene promoter/enhancer, and tested its response to interferon tau (IFN-tau). To address the role of IFN tau in regulating MX1 expression, serial deletion mutants were prepared along with a clone that contained a full-length promoter including the two proximal ISREs but lacking an intronic ISRE site. Promoter deletions showed the two proximal ISRE sites, but not the intronic ISRE site, were required for maximal response to IFN tau. Interestingly, MX1 promoter deletion mutants revealed the presence of distal positive (-920 to -715) and negative (-715 to -437) regulatory regions. Identifying positive and negative regulatory regions in MX1 promoter will help define the complex regulation of MX1 during early pregnancy in ruminants.",
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Cloning and characterizing of the ovine MX1 gene promoter/enhancer region. / Assiri, A. M.; Ott, Troy.

In: Developmental and Comparative Immunology, Vol. 31, No. 8, 13.04.2007, p. 847-857.

Research output: Contribution to journalArticle

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