Cloning and Expression in Saccharomyces cerevisiae of a Synthetic Gene for the Type-I Trophoblast Interferon Ovine Trophoblast Protein-1: Purification and Antiviral Activity

Troy L. Ott, Gino Van Heeke, Howard M. Johnson, Fuller W. Bazer

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Ovine trophoblast protein-1 (oTP-1) is a unique, Type I, trophoblast interferon (IFN) that possesses potent antiviral activity and is thought to be primarily responsible for maternal recognition of pregnancy in sheep. To provide sufficient amounts of protein for detailed studies, a synthetic gene for oTP-1 was designed and assembled in Escherichia coli, subcloned into a yeast expression plasmid, and used to overproduce recombinant oTP-1 in Saccharomyces cerevisiae. Recombinant oTP-1 was purified from soluble yeast extract using sequential ion-exchange and molecular sieve chromatography. Recombinant oTP-1 purified in this fashion exhibited potent antiviral activity (0.6 × 108 U/mg) similar to native oTP-1. This expression system will enable production of large quantities of soluble, biologically active, and correctly processed recombinant oTP-1. Furthermore, the synthetic gene construct facilitates introduction of mutations for ongoing structure/function studies of this unique, Type I, trophoblast IFN.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalJournal of Interferon Research
Volume11
Issue number6
DOIs
StatePublished - Dec 1991

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

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