Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements.

T. Williams, A. Admon, B. Lüscher, R. Tjian

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Abstract

Human AP-2 is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to stimulate transcription of selected genes. Here, we report the isolation and characterization of a human cDNA clone containing the entire protein-coding region of AP-2. The deduced primary amino acid sequence of AP-2 does not contain a domain resembling any previously identified DNA binding motif. However, an interesting feature of the AP-2 protein is a clustered arrangement of proline and glutamine residues that have been found recently within the activation domains of other transcription factors. Expression of the AP-2 clone in bacteria yields a protein that binds to DNA and activates transcription in vitro in a comparable manner to native human AP-2. Transfection of cDNA clones into Drosophila cells indicates that the AP-2 gene product can also activate gene expression in vivo in a DNA template-dependent manner. Expression of endogenous AP-2 is repressed in a hepatoma cell line and stimulated following retinoic-acid-induced differentiation of a human teratocarcinoma cell line. This indicates that AP-2 may be a transcription factor involved in the control of developmentally regulated gene expression.

Original languageEnglish (US)
Pages (from-to)1557-1569
Number of pages13
JournalGenes & development
Volume2
Issue number12 A
DOIs
Publication statusPublished - Dec 1988

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All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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