Cloning and sequencing of cDNAs encoding two SSS

Yunjun Ai, De Sheng Tsai, Teh-hui Kao

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A defective S-allele, S0, and a functional S-allele, Sx, have previously been found to be retained in an F1 hybrid of a self-compatible commercial cultivar of Petunia hybrida. Pistil proteins associated with these two alleles have also been identified. Their amino-terminal sequences have been found to share a high degree of similarity with those of S-proteins characterized from self-incompatible solanaceous species. Here we report the isolation and sequencing of cDNAs encoding S0- and Sx-proteins. Their deduced amino acid sequences contain all the consensus primary structural features of S-proteins from self-incompatible solanaceous species. Both proteins also have ribonuclease activity. The implications of these findings are discussed in relation to the presumed function of the S-protein in the self-incompatibility interaction.

Original languageEnglish (US)
Pages (from-to)523-528
Number of pages6
JournalPlant Molecular Biology
Volume19
Issue number3
DOIs
StatePublished - Jun 1 1992

Fingerprint

Cloning
Protein S
Organism Cloning
molecular cloning
Complementary DNA
Alleles
Petunia
Proteins
proteins
Ribonucleases
alleles
Amino Acid Sequence
Amino Acids
Petunia hybrida
ribonucleases
pistil
amino acid sequences
cultivars

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ai, Yunjun ; Tsai, De Sheng ; Kao, Teh-hui. / Cloning and sequencing of cDNAs encoding two SSS. In: Plant Molecular Biology. 1992 ; Vol. 19, No. 3. pp. 523-528.
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Cloning and sequencing of cDNAs encoding two SSS. / Ai, Yunjun; Tsai, De Sheng; Kao, Teh-hui.

In: Plant Molecular Biology, Vol. 19, No. 3, 01.06.1992, p. 523-528.

Research output: Contribution to journalArticle

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