Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum

A. P. Shuber, E. C. Orr, M. A. Recny, P. F. Schendel, H. D. May, N. L. Schauer, J. G. Ferry

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Abstract

The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed.

Original languageEnglish (US)
Pages (from-to)12942-12947
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number28
StatePublished - 1986

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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