Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli

Marina N. Vassylyeva, Anna A. Perederina, Vladimir Svetlov, Shigeyuki Yokoyama, Irina Artsimovitch, Dmitry G. Vassylyev

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapox-diffusion technique in two different space groups, P212 121 (a = 91.32, b = 96.59, c = 117.48 Å) and C222 (a = 80.6, b = 115.1, c = 149.57 Å). The crystals belonging to space group P212121, improved by macroseeding, diffract beyond 2.0 Å at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P212121 crystal form at the absorption edge for Zn atoms.

Original languageEnglish (US)
Pages (from-to)1611-1613
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number9
DOIs
StatePublished - Sep 1 2004

All Science Journal Classification (ASJC) codes

  • Structural Biology

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