Cloning of a sodium channel α subunit from rabbit Schwann cells

Scott M. Belcher, Cynthia A. Zerillo, Robert Levenson, J. Murdoch Ritchie, James R. Howe

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Abstract

Overlapping cDNA clones spanning the entire coding region of a Na-channel α subunit were isolated from cultured Schwann cells from rabbits. The coding region predicts a polypeptide (Na(s)) of 1984 amino acids exhibiting several features characteristic of Na-channel α subunits isolated from other tissues. Sequence comparisons showed that the Na(s) α subunit resembles most the family of Na channels isolated from brain (≃80% amino acid identity) and is least similar (≃55% amino acid identity) to the atypical Na channel expressed in human heart and the partial rat cDNA, NaG. As for the brain II and III isoforms, two variants of Na(s) exist that appear tu arise by alternative splicing. The results of reverse transcriptase-polymerase chain reaction experiments suggest that expression of Na(s) transcripts is restricted to cells in the peripheral and central nervous systems. Expression was detected in cultured Schwann cells, sciatic nerve, brain, and spinal cord but not in skeletal or cardiac muscle, liver, kidney, or lung.

Original languageEnglish (US)
Pages (from-to)11034-11038
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number24
DOIs
Publication statusPublished - Nov 21 1995

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