Cloning of differential expression fragments in cauliflower after Xanthomonas campestris inoculation

Y. Gu, Yingwei Mao, C. G. Wang, Q. C. Zhao, D. L. Sun, W. Q. Song

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A near isogenic line (NIL) of Brassica oleracea var. botrytis with resistant and susceptible lines C712 and C731, was used in this study. More than 100 differentially expressed cDNA fragments were obtained from black rot resistant cauliflower plants obtained using cDNA-amplified fragment length polymorphism (AFLP) after infection with the pathogen. Thirteen of these fragments were cloned and subjected to reverse Northern blot analysis using both infected and control cDNA pools. Two positive clones, M2 and M6, were isolated. Northern dot blot and Northern blot analyses showed that M2 was constitutively expressed, whereas M6 contained a gene that was differentially expressed during pathogen infection. Moreover, M6 cDNA fragment was also highly expressed 16-24 h after H2O2 treatment. Southern blots showed that M6 is a single copy gene in the cauliflower genome, and encodes a protein with 84 % homology to gene on Arabidopsis chromosome 1. The deduced M6 protein has 91 % positive homology with the Arabidopsis 2A6 protein, which regulates ethylene synthesis; 76 % homology with a 1-aminocyclopropane-1-carboxylate oxidase (ACO), the last enzyme in ethylene synthesis; and 70 % homology with an ethylene induced DNA binding factor. These results suggest that M6 gene fragment is a new H2O2 downstream defense related gene fragment and can be induced by Xanthomonas campestris pv. campestris and H2O2.

Original languageEnglish (US)
Pages (from-to)462-468
Number of pages7
JournalBiologia Plantarum
Volume52
Issue number3
DOIs
StatePublished - Sep 1 2008

Fingerprint

Xanthomonas campestris
cauliflower
molecular cloning
ethylene
genes
Northern blotting
Arabidopsis
Brassica oleracea var. botrytis
Xanthomonas campestris pv. campestris
synthesis
proteins
pathogens
isogenic lines
infection
Southern blotting
amplified fragment length polymorphism
clones
chromosomes
genome
DNA

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Horticulture

Cite this

Gu, Y. ; Mao, Yingwei ; Wang, C. G. ; Zhao, Q. C. ; Sun, D. L. ; Song, W. Q. / Cloning of differential expression fragments in cauliflower after Xanthomonas campestris inoculation. In: Biologia Plantarum. 2008 ; Vol. 52, No. 3. pp. 462-468.
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Cloning of differential expression fragments in cauliflower after Xanthomonas campestris inoculation. / Gu, Y.; Mao, Yingwei; Wang, C. G.; Zhao, Q. C.; Sun, D. L.; Song, W. Q.

In: Biologia Plantarum, Vol. 52, No. 3, 01.09.2008, p. 462-468.

Research output: Contribution to journalArticle

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AU - Mao, Yingwei

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