Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2.

J. A. Lumadue, A. B. Glick, F. H. Ruddle

Research output: Contribution to journalArticle

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Abstract

Among the earliest expressed antigens on the surface of activated human lymphocytes is the surface antigen 4F2. We have used DNA-mediated gene transfer and fluorescence-activated cell sorting to obtain cell lines that contain the gene encoding the large subunit of the human 4F2 antigen in a mouse L-cell background. Human DNAs cloned from these cell lines were subsequently used as hybridization probes to isolate a full-length cDNA clone expressing 4F2. Sequence analysis of the coding region has revealed an amino acid sequence of 529 residues. Hydrophobicity plotting has predicted a probable structure for the protein that includes an external carboxyl terminus, an internal leader sequence, a single hydrophobic transmembrane domain, and two possible membrane-associated domains. The 4F2 cDNA detects a single 1.8-kilobase mRNA in T-cell and B-cell lines. RNA gel blot analysis of RNA derived from quiescent and serum-stimulated Swiss 3T3 fibroblasts reveals a cell-cycle modulation of 4F2 gene expression: the mRNA is present in quiescent fibroblasts but increases 8-fold 24-36 hr after stimulation, at the time of maximal DNA synthesis.

Original languageEnglish (US)
Pages (from-to)9204-9208
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number24
DOIs
StatePublished - Dec 1987

All Science Journal Classification (ASJC) codes

  • General

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