Cloud-point extraction and capillary electrochromatography: An approach for the analysis of selected environmental toxicants in spiked human serum

Sarath R. Sirimanne, John R. Barr, Donald G. Patterson

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The use of cloud-point extraction (CPE) coupled with capillary electrochromatography (CEC) as an approach for the analysis of environmental toxicants in human serum was explored. Human serum samples spiked with polycyclic aromatic hydrocarbons, polychlorinated dibenzo-p-dioxins, or phthalates were extracted using Genapol X-080 and 25% NaCl at 46°C for 5 min. The surfactant-rich phase was treated with acetonitrile to remove some of the interfering coextractants and then analyzed by CEC. The analytes within these three groups were well separated via this technique even though the presence of the residual coextractants/surfactant shifted the migration time slightly. The use of CPE-CEC proved to be a viable approach for environmental toxicant analysis. In addition, both CPE and CEC require relatively small amounts of organic solvents compared with conventional sample preparation techniques, and thus are more environmentally friendly.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalJournal of Microcolumn Separations
Volume11
Issue number2
StatePublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Filtration and Separation

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