Co-ordinated regulation of the extracytoplasmic stress factor, sigmaE, with other Escherichia coli sigma factors by (p)ppGpp and DksA may be achieved by specific regulation of individual holoenzymes

Saumya Gopalkrishnan, Herve Nicoloff, Sarah E. Ades

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The E. coli alternative sigma factor, σE, transcribes genes required to maintain the cell envelope and is activated by conditions that destabilize the envelope. σE is also activated during entry into stationary phase in the absence of envelope stress by the alarmone (p)ppGpp. (p)ppGpp controls a large regulatory network, reducing expression of σ70-dependent genes required for rapid growth and activating σ70-dependent and alternative sigma factor-dependent genes required for stress survival. The DksA protein often potentiates the effects of (p)ppGpp. Here we examine regulation of σE by (p)ppGpp and DksA following starvation for nutrients. We find that (p)ppGpp is required for increased σE activity under all conditions tested, but the requirement for DksA varies. DksA is required during amino acid starvation, but is dispensable during phosphate starvation. In contrast, regulation of σS is (p)ppGpp- and DksA-dependent under all conditions tested, while negative regulation of σ70 is DksA- but not (p)ppGpp-dependent during phosphate starvation, yet requires both factors during amino acid starvation. These findings suggest that the mechanism of transcriptional regulation by (p)ppGpp and/or DksA cannot yet be explained by a unifying model and is specific to individual promoters, individual holoenzymes, and specific starvation conditions.

Original languageEnglish (US)
Pages (from-to)479-493
Number of pages15
JournalMolecular Microbiology
Volume93
Issue number3
DOIs
StatePublished - Aug 2014

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Co-ordinated regulation of the extracytoplasmic stress factor, sigmaE, with other Escherichia coli sigma factors by (p)ppGpp and DksA may be achieved by specific regulation of individual holoenzymes'. Together they form a unique fingerprint.

Cite this