Co-regulation of root hair tip growth by ROP GTPases and nitrogen source modulated pH fluctuations

Daria Bloch, Gabriele Brigitte Monshausen, Simon Gilroy, Shaul Yalovsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Growth of plant cells involves tight regulation of the cytoskeleton and vesicle trafficking by processes including the action of the ROP small G proteins together with pH-modulated cell wall modifications. Yet, little is known on how these systems are coordinated. In a paper recently published in Plant Cell and Environment1 we show that ROPs/ RACs function synergistically with NH4 NO4-modulated pH fluctuations to regulate root hair growth. Root hairs expand exclusively at their apical end in a strictly polarized manner by a process known as tip growth. The highly polarized secretion at the apex is maintained by a complex network of factors including the spatial organization of the actin cytoskeleton, tip-focused ion gradients and by small G proteins. Expression of constitutively active ROP mutants disrupts polar growth, inducing the formation of swollen root hairs. Root hairs are also known to elongate in an oscillating manner, which is correlated with oscillatory H+ fluxes at the tip. Our analysis shows that root hair elongation in wild type plants and swelling in transgenic plants expressing a constitutively active ROP11 (rop11CA) is sensitive to the presence of NH4+ at concentrations higher than 1 mM and on NO3-. The NH4+ and NO3- ions did not affect the localization of ROP in the membrane but modulated pH fluctuations at the root hair tip. Actin organization and reactive oxygen species distribution were abnormal in rop11CA root hairs but were similar to wild type root hairs when seedlings were grownon medium lacking NH4+ and/or NO3+. These observations suggest that the nitrogen source-modulated pH fluctuations may function synergistically with ROP regulated signaling during root hair tip growth. Interestingly, under certain growth conditions, expression of rop11CA suppressed ammoniumtoxicity, similar to auxin resistant mutants. In this short review we discuss these findings and their implications.

Original languageEnglish (US)
Pages (from-to)426-429
Number of pages4
JournalPlant Signaling and Behavior
Volume6
Issue number3
DOIs
StatePublished - Mar 1 2011

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guanosinetriphosphatase
root hairs
nitrogen
G-proteins
ions
mutants
cytoskeleton
microfilaments
actin
transgenic plants
reactive oxygen species
auxins
biogeography
cell walls
plant growth
secretion
cells
seedlings

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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title = "Co-regulation of root hair tip growth by ROP GTPases and nitrogen source modulated pH fluctuations",
abstract = "Growth of plant cells involves tight regulation of the cytoskeleton and vesicle trafficking by processes including the action of the ROP small G proteins together with pH-modulated cell wall modifications. Yet, little is known on how these systems are coordinated. In a paper recently published in Plant Cell and Environment1 we show that ROPs/ RACs function synergistically with NH4 NO4-modulated pH fluctuations to regulate root hair growth. Root hairs expand exclusively at their apical end in a strictly polarized manner by a process known as tip growth. The highly polarized secretion at the apex is maintained by a complex network of factors including the spatial organization of the actin cytoskeleton, tip-focused ion gradients and by small G proteins. Expression of constitutively active ROP mutants disrupts polar growth, inducing the formation of swollen root hairs. Root hairs are also known to elongate in an oscillating manner, which is correlated with oscillatory H+ fluxes at the tip. Our analysis shows that root hair elongation in wild type plants and swelling in transgenic plants expressing a constitutively active ROP11 (rop11CA) is sensitive to the presence of NH4+ at concentrations higher than 1 mM and on NO3-. The NH4+ and NO3- ions did not affect the localization of ROP in the membrane but modulated pH fluctuations at the root hair tip. Actin organization and reactive oxygen species distribution were abnormal in rop11CA root hairs but were similar to wild type root hairs when seedlings were grownon medium lacking NH4+ and/or NO3+. These observations suggest that the nitrogen source-modulated pH fluctuations may function synergistically with ROP regulated signaling during root hair tip growth. Interestingly, under certain growth conditions, expression of rop11CA suppressed ammoniumtoxicity, similar to auxin resistant mutants. In this short review we discuss these findings and their implications.",
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Co-regulation of root hair tip growth by ROP GTPases and nitrogen source modulated pH fluctuations. / Bloch, Daria; Monshausen, Gabriele Brigitte; Gilroy, Simon; Yalovsky, Shaul.

In: Plant Signaling and Behavior, Vol. 6, No. 3, 01.03.2011, p. 426-429.

Research output: Contribution to journalArticle

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