Codependency of H2B monoubiquitination and nucleosome reassembly on Chdl

Jung Shin Lee, Alexander S. Garrett, Kuangyu Yen, Yoh Hei Takahashi, Deqing Hu, Jessica Jackson, Christopher Seidel, B. Franklin Pugh, Ali Shilatifard

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Monoubiquitination of histone H2B on Lys 123 (H2BK123ub) is a transient histone modification considered to be essential for establishing H3K4 and H3K79 trimethylation by Set1/COMPASS and Dot1, respectively. Here, we identified Chdl as a factor that is required for the maintenance of high levels of H2B monoubiquitination, but not for H3K4 and H3K79 trimethylation. Loss of Chdl results in a substantial loss of H2BK123ub levels with little to no effect on the genome-wide pattern of H3K4 and H3K79 trimethylation. Our data show that nucleosomal occupancy is reduced in gene bodies in both chd1Δ and, as has been shown, K123A mutant backgrounds. We also demonstrated that Chdl's function in maintaining H2BK123ub levels is conserved from yeast to humans. Our study provides evidence that only small levels of H2BK123ub are necessary for full levels of H3K4 and H3K79 trimethylation in vivo and points to a possible role for Chdl in positively regulating gene expression through promoting nucleosome reassembly coupled with H2B monoubiquitination.

Original languageEnglish (US)
Pages (from-to)914-919
Number of pages6
JournalGenes and Development
Volume26
Issue number9
DOIs
StatePublished - May 1 2012

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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    Lee, J. S., Garrett, A. S., Yen, K., Takahashi, Y. H., Hu, D., Jackson, J., Seidel, C., Franklin Pugh, B., & Shilatifard, A. (2012). Codependency of H2B monoubiquitination and nucleosome reassembly on Chdl. Genes and Development, 26(9), 914-919. https://doi.org/10.1101/gad.186841.112