Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain

Rae Silver, P. Witkovsky, P. Horvath, V. Alones, Colin Barnstable, M. N. Lehman

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Cerebrospinal fluid-contacting (CSF) cells in both the septal and the tuberal areas in the brain of the ring dove are labeled by RET-P1, a monoclonal antibody to opsin that reacts with inner and outer segment membranes of rod photoreceptors in a variety of vertebrates. Immunoblot analysis of proteins from diverse brain regions, however, revealed bands of anti-RET-P1 immunoreactivity that did not correspond to opsin. Binding of RET-P1 to opsin-containing membranes, was not inhibited by membranes rich in muscarinic and β-adrenergic receptor proteins (red blood cells, heart, lung) taken from doves. RET-P1-immunoreactive CSF-contacting cells emit a dendritic process that penetrates the ependyma and ends in a knob-like terminal suspended in the ventricle. These cells also possess other processes that penetrate more or less deeply into the neuropil. Additionally, a band of labeled fibers occurs in the external layer of the median eminence. A double-label technique demonstrated that RET-P1-positive cells coexpress VIP-like immunoreactivity. VIP-positive cells in other brain areas are not RET-P1-positive.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalCell And Tissue Research
Volume253
Issue number1
DOIs
StatePublished - Jul 1 1988

Fingerprint

Opsins
Cerebrospinal Fluid
Neurons
Brain
Membranes
Ependyma
Septum of Brain
Retinal Rod Photoreceptor Cells
Median Eminence
Neuropil
Columbidae
Muscarinic Receptors
Adrenergic Receptors
Vertebrates
Proteins
Erythrocytes
Monoclonal Antibodies
Lung

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Silver, Rae ; Witkovsky, P. ; Horvath, P. ; Alones, V. ; Barnstable, Colin ; Lehman, M. N. / Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain. In: Cell And Tissue Research. 1988 ; Vol. 253, No. 1. pp. 189-198.
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Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain. / Silver, Rae; Witkovsky, P.; Horvath, P.; Alones, V.; Barnstable, Colin; Lehman, M. N.

In: Cell And Tissue Research, Vol. 253, No. 1, 01.07.1988, p. 189-198.

Research output: Contribution to journalArticle

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