Cold stress causes rapid but differential changes in properties of plasma membrane H+-ATPase of camelina and rapeseed

Hyun Sung Kim, Jung Min Oh, Sheng Luan, John E. Carlson, Sung Ju Ahn

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Camelina (Camelina sativa) and rapeseed (Brassica napus) are well-established oil-seed crops with great promise also for biofuels. Both are cold-tolerant, and camelina is regarded to be especially appropriate for production on marginal lands. We examined physiological and biochemical alterations in both species during cold stress treatment for 3 days and subsequent recovery at the temperature of 25°C for 0, 0.25, 0.5, 1, 2, 6, and 24h, with particular emphasis on the post-translational regulation of the plasma membrane (PM) H+-ATPase (EC3.6.3.14). The activity and translation of the PM H+-ATPase, as well as 14-3-3 proteins, increased after 3 days of cold stress in both species but recovery under normal conditions proceeded differently. The increase in H+-ATPase activity was the most dramatic in camelina roots after recovery for 2h at 25°C, followed by decay to background levels within 24h. In rapeseed, the change in H+-ATPase activity during the recovery period was less pronounced. Furthermore, H+-pumping increased in both species after 15min recovery, but to twice the level in camelina roots compared to rapeseed. Protein gel blot analysis with phospho-threonine anti-bodies showed that an increase in phosphorylation levels paralleled the increase in H+-transport rate. Thus our results suggest that cold stress and recovery in camelina and rapeseed are associated with PM H+-fluxes that may be regulated by specific translational and post-translational modifications.

Original languageEnglish (US)
Pages (from-to)828-837
Number of pages10
JournalJournal of Plant Physiology
Volume170
Issue number9
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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