Collaborative non-self recognition system in S-RNase-based self-incompatibility

Ken Ichi Kubo, Tetsuyuki Entani, Akie Takara, Ning Wang, Allison M. Fields, Zhihua Hua, Mamiko Toyoda, Shin Ichi Kawashima, Toshio Ando, Akira Isogai, Teh Hui Kao, Seiji Takayama

Research output: Contribution to journalArticlepeer-review

175 Scopus citations

Abstract

Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin - 26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants.

Original languageEnglish (US)
Pages (from-to)796-799
Number of pages4
JournalScience
Volume330
Issue number6005
DOIs
StatePublished - Nov 5 2010

All Science Journal Classification (ASJC) codes

  • General

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