Collagens in an adult bovine medial collateral ligament: Immunofluorescence localization by confocal microscopy reveals that type XIV collagen predominates at the ligament-bone junction

Christopher Niyibizi, Camilla Sagarriga Visconti, Karl Kavalkovich, Savio L.Y. Woo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To understand the structure and function of medial collateral ligament, collagens present in an adult bovine ligament were determined. The mid-section of the ligament was powdered and extracted with 4M guanidinium hydrochloride, and the residue was digested with pepsin to solubilize the collagens. Type I collagen was the major fibril collagen recovered in the pepsin solubilized fraction, with types III and V each representing about 5% and 2%, respectively. Type VI collagen was the major collagen present in the guanidinium hydrochloride extract, and it accounted for about 40% of the proteins in the extract or 4% of the tissue dry weight. Type XII and XIV collagens were also detected in the guanadinium hydrochloride extract as minor components. Immunofluorescence localization using confocal microscopy showed that type XII and XIV collagens are associated with the ligament fibrillar network and that type XIV collagen was prominent at the ligament-bone junction. These data reinforce the notion that these collagens are associated with the type I collagen fibrillar network in connective tissues. In view of high mechanical stresses that exist at the ligament-bone interface, presence of type XIV collagen in high concentration at this junction may contribute to the modulation of the biomechanical properties of this tissue.

Original languageEnglish (US)
Pages (from-to)743-751
Number of pages9
JournalMatrix Biology
Volume14
Issue number9
DOIs
StatePublished - Dec 1995

Fingerprint

Collateral Ligaments
Ligaments
Confocal Microscopy
Fluorescent Antibody Technique
Collagen
Bone and Bones
Collagen Type XII
Pepsin A
Guanidine
Collagen Type I
Collagen Type VI
Mechanical Stress
Connective Tissue
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

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title = "Collagens in an adult bovine medial collateral ligament: Immunofluorescence localization by confocal microscopy reveals that type XIV collagen predominates at the ligament-bone junction",
abstract = "To understand the structure and function of medial collateral ligament, collagens present in an adult bovine ligament were determined. The mid-section of the ligament was powdered and extracted with 4M guanidinium hydrochloride, and the residue was digested with pepsin to solubilize the collagens. Type I collagen was the major fibril collagen recovered in the pepsin solubilized fraction, with types III and V each representing about 5{\%} and 2{\%}, respectively. Type VI collagen was the major collagen present in the guanidinium hydrochloride extract, and it accounted for about 40{\%} of the proteins in the extract or 4{\%} of the tissue dry weight. Type XII and XIV collagens were also detected in the guanadinium hydrochloride extract as minor components. Immunofluorescence localization using confocal microscopy showed that type XII and XIV collagens are associated with the ligament fibrillar network and that type XIV collagen was prominent at the ligament-bone junction. These data reinforce the notion that these collagens are associated with the type I collagen fibrillar network in connective tissues. In view of high mechanical stresses that exist at the ligament-bone interface, presence of type XIV collagen in high concentration at this junction may contribute to the modulation of the biomechanical properties of this tissue.",
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Collagens in an adult bovine medial collateral ligament : Immunofluorescence localization by confocal microscopy reveals that type XIV collagen predominates at the ligament-bone junction. / Niyibizi, Christopher; Visconti, Camilla Sagarriga; Kavalkovich, Karl; Woo, Savio L.Y.

In: Matrix Biology, Vol. 14, No. 9, 12.1995, p. 743-751.

Research output: Contribution to journalArticle

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AU - Woo, Savio L.Y.

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