Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization

Lin He, Michael D. Musick, Sheila R. Nicewarner, Frank G. Salinas, Stephen Benkovic, Michael J. Natan, Christine Dolan Keating

Research output: Contribution to journalArticle

897 Citations (Scopus)

Abstract

A new approach to ultrasensitive detection of DNA hybridization based on nanoparticle-amplified surface plasmon resonance (SPR) is described. Use of the Au nanoparticle tags leads to a greater than 10-fold increase in angle shift, corresponding to a more than 1000-fold improvement in sensitivity for the target oligonucleotide as compared to the unamplified binding event. This enhanced shift in SPR reflectivity is a combined result of greatly increased surface mass, high dielectric constant of Au particles, and electromagnetic coupling between Au nanoparticles and the Au film. DNA melting and digestion experiments further supported the feasibility of this approach in DNA hybridization studies. The extremely large angle shifts observed in particle-amplified SPR make it possible to conduct SPR imaging experiments on DNA arrays. In the present work, macroscopic 4 x 4 arrays were employed, and a ~ 10 pM limit of quantitation was achieved for 24-mer oligonucleotides (surface density ≤ 8 x 108 molecules/cm2). Even without further optimization, the sensitivity of this technique begins to approach that of traditional fluorescence-based methods for DNA hybridization. These results illustrate the potential of particle-amplified SPR for array-based DNA analysis and ultrasensitive detection of oligonucleotides.

Original languageEnglish (US)
Pages (from-to)9071-9077
Number of pages7
JournalJournal of the American Chemical Society
Volume122
Issue number38
DOIs
StatePublished - Sep 27 2000

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
DNA
Oligonucleotides
Nanoparticles
Oligonucleotide Array Sequence Analysis
Nucleic Acid Denaturation
Electromagnetic Phenomena
Electromagnetic coupling
Digestion
Fluorescence
Melting
Permittivity
Experiments
Imaging techniques
Molecules

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

He, Lin ; Musick, Michael D. ; Nicewarner, Sheila R. ; Salinas, Frank G. ; Benkovic, Stephen ; Natan, Michael J. ; Keating, Christine Dolan. / Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization. In: Journal of the American Chemical Society. 2000 ; Vol. 122, No. 38. pp. 9071-9077.
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Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization. / He, Lin; Musick, Michael D.; Nicewarner, Sheila R.; Salinas, Frank G.; Benkovic, Stephen; Natan, Michael J.; Keating, Christine Dolan.

In: Journal of the American Chemical Society, Vol. 122, No. 38, 27.09.2000, p. 9071-9077.

Research output: Contribution to journalArticle

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