Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations

Zora Djuric, Christine M. Bassis, Melissa A. Plegue, Jianwei Ren, Rena Chan, El Khansa Sidahmed, D. Kim Turgeon, Mack Ruffin, Ikuko Kato, Ananda Sen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Relatively high serum carotenoid levels are associated with reduced risks of chronic diseases, but inter-individual variability in serum carotenoid concentrations is modestly explained by diet. The bacterial community in the colon could contribute to the bioaccessibility of carotenoids by completing digestion of plant cells walls and by modulating intestinal permeability. Objective: To evaluate whether colonic bacterial composition is associated with serum and colon carotenoid concentrations. Design: The study was a randomized dietary intervention trial in healthy individuals who were at increased risk of colon cancer. Colon mucosal biopsy samples were obtained before and after 6 months of intervention without prior preparation of the bowels. Participants/setting: Participants were recruited from Ann Arbor, MI, and nearby areas from July 2007 to November 2010. Biopsy data were available from 88 participants at baseline and 82 participants after 6 months. Methods: Study participants were randomized to counseling for either a Mediterranean diet or a Healthy Eating diet for 6 months. Results: At baseline, bacterial communities in biopsy samples from study participants in the highest vs the lowest tertile of total serum carotenoid levels differed by several parameters. Linear discriminant analysis effect size identified 11 operational taxonomic units that were significantly associated with higher serum carotenoid levels. In linear regression analyses, three of these accounted for an additional 12% of the variance in serum total carotenoid concentrations after including body mass index, smoking, and dietary intakes in the model. These factors together explained 36% of the inter-individual variance in serum total carotenoid concentrations. The bacterial community in the colonic mucosa, however, was resistant to change after dietary intervention with either a Mediterranean diet or Healthy Eating diet, each of which doubled fruit and vegetable intakes. Conclusions: The colonic mucosal bacterial community was associated with serum carotenoid concentrations at baseline but was not appreciably changed by dietary intervention.

Original languageEnglish (US)
Pages (from-to)606-616.e3
JournalJournal of the Academy of Nutrition and Dietetics
Volume118
Issue number4
DOIs
StatePublished - Apr 1 2018

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Carotenoids
carotenoids
Bacteria
bacteria
Serum
bacterial communities
colon
Mediterranean Diet
biopsy
Mediterranean diet
Colon
healthy diet
Biopsy
diet
counseling
smoking (food products)
vegetable consumption
Plant Cells
fruit consumption
Discriminant Analysis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

Cite this

Djuric, Zora ; Bassis, Christine M. ; Plegue, Melissa A. ; Ren, Jianwei ; Chan, Rena ; Sidahmed, El Khansa ; Turgeon, D. Kim ; Ruffin, Mack ; Kato, Ikuko ; Sen, Ananda. / Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations. In: Journal of the Academy of Nutrition and Dietetics. 2018 ; Vol. 118, No. 4. pp. 606-616.e3.
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title = "Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations",
abstract = "Background: Relatively high serum carotenoid levels are associated with reduced risks of chronic diseases, but inter-individual variability in serum carotenoid concentrations is modestly explained by diet. The bacterial community in the colon could contribute to the bioaccessibility of carotenoids by completing digestion of plant cells walls and by modulating intestinal permeability. Objective: To evaluate whether colonic bacterial composition is associated with serum and colon carotenoid concentrations. Design: The study was a randomized dietary intervention trial in healthy individuals who were at increased risk of colon cancer. Colon mucosal biopsy samples were obtained before and after 6 months of intervention without prior preparation of the bowels. Participants/setting: Participants were recruited from Ann Arbor, MI, and nearby areas from July 2007 to November 2010. Biopsy data were available from 88 participants at baseline and 82 participants after 6 months. Methods: Study participants were randomized to counseling for either a Mediterranean diet or a Healthy Eating diet for 6 months. Results: At baseline, bacterial communities in biopsy samples from study participants in the highest vs the lowest tertile of total serum carotenoid levels differed by several parameters. Linear discriminant analysis effect size identified 11 operational taxonomic units that were significantly associated with higher serum carotenoid levels. In linear regression analyses, three of these accounted for an additional 12{\%} of the variance in serum total carotenoid concentrations after including body mass index, smoking, and dietary intakes in the model. These factors together explained 36{\%} of the inter-individual variance in serum total carotenoid concentrations. The bacterial community in the colonic mucosa, however, was resistant to change after dietary intervention with either a Mediterranean diet or Healthy Eating diet, each of which doubled fruit and vegetable intakes. Conclusions: The colonic mucosal bacterial community was associated with serum carotenoid concentrations at baseline but was not appreciably changed by dietary intervention.",
author = "Zora Djuric and Bassis, {Christine M.} and Plegue, {Melissa A.} and Jianwei Ren and Rena Chan and Sidahmed, {El Khansa} and Turgeon, {D. Kim} and Mack Ruffin and Ikuko Kato and Ananda Sen",
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Djuric, Z, Bassis, CM, Plegue, MA, Ren, J, Chan, R, Sidahmed, EK, Turgeon, DK, Ruffin, M, Kato, I & Sen, A 2018, 'Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations', Journal of the Academy of Nutrition and Dietetics, vol. 118, no. 4, pp. 606-616.e3. https://doi.org/10.1016/j.jand.2017.09.013

Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations. / Djuric, Zora; Bassis, Christine M.; Plegue, Melissa A.; Ren, Jianwei; Chan, Rena; Sidahmed, El Khansa; Turgeon, D. Kim; Ruffin, Mack; Kato, Ikuko; Sen, Ananda.

In: Journal of the Academy of Nutrition and Dietetics, Vol. 118, No. 4, 01.04.2018, p. 606-616.e3.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations

AU - Djuric, Zora

AU - Bassis, Christine M.

AU - Plegue, Melissa A.

AU - Ren, Jianwei

AU - Chan, Rena

AU - Sidahmed, El Khansa

AU - Turgeon, D. Kim

AU - Ruffin, Mack

AU - Kato, Ikuko

AU - Sen, Ananda

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Background: Relatively high serum carotenoid levels are associated with reduced risks of chronic diseases, but inter-individual variability in serum carotenoid concentrations is modestly explained by diet. The bacterial community in the colon could contribute to the bioaccessibility of carotenoids by completing digestion of plant cells walls and by modulating intestinal permeability. Objective: To evaluate whether colonic bacterial composition is associated with serum and colon carotenoid concentrations. Design: The study was a randomized dietary intervention trial in healthy individuals who were at increased risk of colon cancer. Colon mucosal biopsy samples were obtained before and after 6 months of intervention without prior preparation of the bowels. Participants/setting: Participants were recruited from Ann Arbor, MI, and nearby areas from July 2007 to November 2010. Biopsy data were available from 88 participants at baseline and 82 participants after 6 months. Methods: Study participants were randomized to counseling for either a Mediterranean diet or a Healthy Eating diet for 6 months. Results: At baseline, bacterial communities in biopsy samples from study participants in the highest vs the lowest tertile of total serum carotenoid levels differed by several parameters. Linear discriminant analysis effect size identified 11 operational taxonomic units that were significantly associated with higher serum carotenoid levels. In linear regression analyses, three of these accounted for an additional 12% of the variance in serum total carotenoid concentrations after including body mass index, smoking, and dietary intakes in the model. These factors together explained 36% of the inter-individual variance in serum total carotenoid concentrations. The bacterial community in the colonic mucosa, however, was resistant to change after dietary intervention with either a Mediterranean diet or Healthy Eating diet, each of which doubled fruit and vegetable intakes. Conclusions: The colonic mucosal bacterial community was associated with serum carotenoid concentrations at baseline but was not appreciably changed by dietary intervention.

AB - Background: Relatively high serum carotenoid levels are associated with reduced risks of chronic diseases, but inter-individual variability in serum carotenoid concentrations is modestly explained by diet. The bacterial community in the colon could contribute to the bioaccessibility of carotenoids by completing digestion of plant cells walls and by modulating intestinal permeability. Objective: To evaluate whether colonic bacterial composition is associated with serum and colon carotenoid concentrations. Design: The study was a randomized dietary intervention trial in healthy individuals who were at increased risk of colon cancer. Colon mucosal biopsy samples were obtained before and after 6 months of intervention without prior preparation of the bowels. Participants/setting: Participants were recruited from Ann Arbor, MI, and nearby areas from July 2007 to November 2010. Biopsy data were available from 88 participants at baseline and 82 participants after 6 months. Methods: Study participants were randomized to counseling for either a Mediterranean diet or a Healthy Eating diet for 6 months. Results: At baseline, bacterial communities in biopsy samples from study participants in the highest vs the lowest tertile of total serum carotenoid levels differed by several parameters. Linear discriminant analysis effect size identified 11 operational taxonomic units that were significantly associated with higher serum carotenoid levels. In linear regression analyses, three of these accounted for an additional 12% of the variance in serum total carotenoid concentrations after including body mass index, smoking, and dietary intakes in the model. These factors together explained 36% of the inter-individual variance in serum total carotenoid concentrations. The bacterial community in the colonic mucosa, however, was resistant to change after dietary intervention with either a Mediterranean diet or Healthy Eating diet, each of which doubled fruit and vegetable intakes. Conclusions: The colonic mucosal bacterial community was associated with serum carotenoid concentrations at baseline but was not appreciably changed by dietary intervention.

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