Colonization and internalization of salmonella enterica in tomato plants

Jie Zheng, Sarah Allard, Sara Reynolds, Patricia Millner, Gabriela Arce, Robert J. Blodgett, Eric Wayne Brown

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The consumption of fresh tomatoes has been linked to numerous food-borne outbreaks involving various serovars of Salmonella enterica. Recent advances in our understanding of plant-microbe interactions have shown that human enteric pathogenic bacteria, including S. enterica, are adapted to survive in the plant environment. In this study, tomato plants (Solanum lycopersicum cv. Micro-Tom) grown in sandy loam soil from Virginia's eastern shore (VES) were inoculated with S. enterica serovars to evaluate plausible internalization routes and to determine if there is any niche fitness for certain serovars. Both infested soil and contaminated blossoms can lead to low internal levels of fruit contamination with Salmonella. Salmonella serovars demonstrated a great ability to survive in environments under tomato cultivation, not only in soil but also on different parts of the tomato plant. Of the five serovars investigated, Salmonella enterica serovars Newport and Javiana were dominant in sandy loam soil, while Salmonella enterica serovars Montevideo and Newport were more prevalent on leaves and blossoms. It was also observed that Salmonella enterica serovar Typhimurium had a poor rate of survival in all the plant parts examined here, suggesting that post-harvest contamination routes are more likely in S. Typhimurium contamination of tomato fruit. Conversely, S. Newport was the most prevalent serovar recovered in both the tomato rhizosphere and phyllosphere. Plants that were recently transplanted (within 3 days) had an increase in observable internalized bacteria, suggesting that plants were more susceptible to internalization right after transplant. These findings suggest that the particular Salmonella serovar and the growth stage of the plant were important factors for internalization through the root system.

Original languageEnglish (US)
Pages (from-to)2494-2502
Number of pages9
JournalApplied and environmental microbiology
Volume79
Issue number8
DOIs
StatePublished - Apr 1 2013

Fingerprint

Salmonella enterica
Lycopersicon esculentum
serotypes
colonization
tomatoes
Salmonella Newport
Salmonella
Soil
sandy loam soils
Salmonella Javiana
sandy loam
Salmonella Montevideo
fruit
phyllosphere
fruits
Fruit
bacteria
Solanum lycopersicum
bacterium
Serogroup

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Zheng, J., Allard, S., Reynolds, S., Millner, P., Arce, G., Blodgett, R. J., & Brown, E. W. (2013). Colonization and internalization of salmonella enterica in tomato plants. Applied and environmental microbiology, 79(8), 2494-2502. https://doi.org/10.1128/AEM.03704-12
Zheng, Jie ; Allard, Sarah ; Reynolds, Sara ; Millner, Patricia ; Arce, Gabriela ; Blodgett, Robert J. ; Brown, Eric Wayne. / Colonization and internalization of salmonella enterica in tomato plants. In: Applied and environmental microbiology. 2013 ; Vol. 79, No. 8. pp. 2494-2502.
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Zheng, J, Allard, S, Reynolds, S, Millner, P, Arce, G, Blodgett, RJ & Brown, EW 2013, 'Colonization and internalization of salmonella enterica in tomato plants', Applied and environmental microbiology, vol. 79, no. 8, pp. 2494-2502. https://doi.org/10.1128/AEM.03704-12

Colonization and internalization of salmonella enterica in tomato plants. / Zheng, Jie; Allard, Sarah; Reynolds, Sara; Millner, Patricia; Arce, Gabriela; Blodgett, Robert J.; Brown, Eric Wayne.

In: Applied and environmental microbiology, Vol. 79, No. 8, 01.04.2013, p. 2494-2502.

Research output: Contribution to journalArticle

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Zheng J, Allard S, Reynolds S, Millner P, Arce G, Blodgett RJ et al. Colonization and internalization of salmonella enterica in tomato plants. Applied and environmental microbiology. 2013 Apr 1;79(8):2494-2502. https://doi.org/10.1128/AEM.03704-12