Combined SP-A-bleomycin effect on cytokines by THP-1 cells: Impact of surfactant lipids on this effect

Weixiong Huang, Guirong Wang, David S. Phelps, Hamid Al-Mondhiry, Joanna Floros

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Surfactant protein A (SP-A) plays a role in host defense and inflammation in the lung. In the present study, we investigated the hypothesis that SP-A is involved in bleomycin-induced pulmonary fibrosis. We studied the effects of human SP-A on bleomycin-induced cytokine production and mRNA expression in THP-1 macrophage-like cells and obtained the following results. 1) Bleomycin-treated THP-1 cells increased tumor necrosis factor (TNF)-α, interleukin (IL)-8, and IL-1β production in dose- and time-dependent patterns, as we have observed with SP-A. TNF-α levels were unaffected by treatment with cytosine arabinoside. 2) The combined bleomycin-SP-A effect on cytokine production is additive by RNase protection assay and synergistic by enzyme-linked immunosorbent assay. 3) Although the bleomycin effect on cytokine production was not significantly affected by the presence of surfactant lipid, the additive and synergistic effect of SP-A-bleomycin on cytokine production was significantly reduced. We speculate that the elevated cytokine levels resulting from the bleomycin-SP-A synergism are responsible for bleomycin-induced pulmonary fibrosis and that surfactant lipids can help ameliorate pulmonary complications observed during bleomycin chemotherapy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume283
Issue number1 27-1
StatePublished - Jul 24 2002

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Pulmonary Surfactant-Associated Protein A
Bleomycin
Surface-Active Agents
Cytokines
Lipids
Pulmonary Fibrosis
Tumor Necrosis Factor-alpha
Pulmonary Surfactants
Cytarabine
Ribonucleases
Interleukin-8
Interleukin-1
Pneumonia
Enzyme-Linked Immunosorbent Assay
Macrophages
Drug Therapy
Lung
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

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abstract = "Surfactant protein A (SP-A) plays a role in host defense and inflammation in the lung. In the present study, we investigated the hypothesis that SP-A is involved in bleomycin-induced pulmonary fibrosis. We studied the effects of human SP-A on bleomycin-induced cytokine production and mRNA expression in THP-1 macrophage-like cells and obtained the following results. 1) Bleomycin-treated THP-1 cells increased tumor necrosis factor (TNF)-α, interleukin (IL)-8, and IL-1β production in dose- and time-dependent patterns, as we have observed with SP-A. TNF-α levels were unaffected by treatment with cytosine arabinoside. 2) The combined bleomycin-SP-A effect on cytokine production is additive by RNase protection assay and synergistic by enzyme-linked immunosorbent assay. 3) Although the bleomycin effect on cytokine production was not significantly affected by the presence of surfactant lipid, the additive and synergistic effect of SP-A-bleomycin on cytokine production was significantly reduced. We speculate that the elevated cytokine levels resulting from the bleomycin-SP-A synergism are responsible for bleomycin-induced pulmonary fibrosis and that surfactant lipids can help ameliorate pulmonary complications observed during bleomycin chemotherapy.",
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Combined SP-A-bleomycin effect on cytokines by THP-1 cells : Impact of surfactant lipids on this effect. / Huang, Weixiong; Wang, Guirong; Phelps, David S.; Al-Mondhiry, Hamid; Floros, Joanna.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 283, No. 1 27-1, 24.07.2002.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Combined SP-A-bleomycin effect on cytokines by THP-1 cells

T2 - Impact of surfactant lipids on this effect

AU - Huang, Weixiong

AU - Wang, Guirong

AU - Phelps, David S.

AU - Al-Mondhiry, Hamid

AU - Floros, Joanna

PY - 2002/7/24

Y1 - 2002/7/24

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