Combined vaccination with different type vaccines of Plasmodium falciparum AMAl

Xun Li, Cai Fang Xue, Jun Miao, Shu Mei Li, Zhong Xiang Liu, Jun Chuan Lei, Xian Feng Wang

Research output: Contribution to journalArticle

Abstract

Objective: To explore the potential of combined vaccination using DNA vaccine, recorabinant vaccinia virus vaccine and recombinant protein vaccine in inducing protective antibodies to malaria antigen AMA1. Methods: AMA1 ectodomain encoding gene was amplified by PCR from the genome of YN strain of Plasmodium falciparum. DNA vaccine plasmid VR1020/E(vaccine D), recombinant modified vaccinia virus Ankara rMVA/E(vaccine V) and prokaryotic expressed AMA1 ectodomain protein E(vaccine P) were constructed and prepared. BALB/c mice were primed two times with vaccine D only or together with GM-CSF expressing plasmid at O w and 3 w, at 9 w they were given a booster dose of vaccine V or vaccine P, at 15 w two groups received another booster with different type vaccines. New Zealand rabbits were immunized under vaccine D-V approach. The titers of IgG in mice sera and the IgG subtypes were determined by ELISA. The ability of immune sera in inhibiting plasmodium merozoites invasion were evaluated. Results: AMA1 specific antibodies in vaccine D primed mice were greatly enhanced after vaccination with vaccine V or vaccine P, their IgG levels increased by 15 to 137 times. The antibody responses can be promoted in mice immunization by vaccine D-V with additional pcDNA3/GM-CSF. Obvious antibodies were induced in rabbits under vaccine D-V approach. Both mice and rabbit immune sera showed remarkable inhibitory effect on the invasion of merozoites into RBCs. Conclusion: It is a potent way to induce protective antibodies to the intraerythrocytic malaria parasites by combined vaccination of DNA vaccine, recombinant MVA vaccine and recombinant protein vaccine.

Original languageEnglish (US)
Pages (from-to)949-952
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume25
Issue number11
StatePublished - Nov 30 2005

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Microbiology (medical)

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  • Cite this

    Li, X., Xue, C. F., Miao, J., Li, S. M., Liu, Z. X., Lei, J. C., & Wang, X. F. (2005). Combined vaccination with different type vaccines of Plasmodium falciparum AMAl. Chinese Journal of Microbiology and Immunology, 25(11), 949-952.